Size and Cutting Pattern of Explant for Micropropagation of Musa (AA Group) ‘Kluai Khai Kasetsart 2’

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พรนัชชา คงพันธุ์
อารยา อาจเจริญ เทียนหอม
ทัศไนย จารุวัฒนพันธ์


Effects of explant size and cutting pattern of Musa (AA group) ‘Kluai Khai Kasetsart 2’ in vitro culture were studied. Two sucker sizes were used: 1 (1 x 1 x 1 cm) and 1.5 (1.5 x 1.5 x 1.5) cm. Both sized suckers were not divided and divided in to 2 and 4 pieces then the explants were cultured on MS medium supplemented with 5 mg/L BA. Intact explant size 1.5 cm was used as a control. The experiment was arranged in a completely randomized design with 10 replications. The result showed that sucker size and cutting pattern affected plantlet survival percentages. Explants size 1.5 cm with 2- and 4-piece cut had survival rate at 60 and 50 %, respectively, which were higher than that of the control (35%), while explant size 1 cm with all cuts had 80% survival rate. The explants size 1 cm with 2- and 4-piece cuts and size 1.5 cm with 4-piece cut gave the highest new shoot numbers (72, 77 and 66 shoots, respectively). Subsequently, the size 1 cm explants were pinched at the terminal bud by scooping method. The pinched explants had higher regenerated shoots (29) than the non-pinched ones (12).


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พรนัชชา คงพันธุ์, คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

อารยา อาจเจริญ เทียนหอม

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

ทัศไนย จารุวัฒนพันธ์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900


[1] Silayoi, B. and Kriengyakul, K., 2001, Mutation inductrion of Kluai Khai using gamma rays through tissue culture, pp. 197-202, 12th Genetics Conference: Gene Revolution Era, Kasetsart University, Bangkok. (in Thai)
[2] Silayoi, B., 2015, Banana, KU Publishing, Bangkok, 512 p. (in Thai)
[3] Department of Agriculture, 2013, Banana Research Development Strategy 2016-2020, Available Source:, March 29, 2018. (in Thai)
[4] Rungrueangkhaoornlertkul, S., 1993, Study of Shoot of 8 Varieties of Bananas by Tissue Culture, Department of Horti culture, Graduate School, Kasetsart University, Bangkok. (in Thai)
[5] El Boullani, R., Lagram, K., El Mousadik, A. and Serghini, M.A., 2017, Effect of explants density and size on the in vitro proliferation and growth of separated shoots of globe artichoke (Cynara cardunculus var. scolymus L.), J. Mater. Environ. Sci. 8: 2469-2473.
[6] Josephine, A.U. and Julian, O., 2011, Split crown technique for mass propagation of smooth cayenne pineapple in South-South Nigeria, Afr. J. Plant Sci. 5: 591-598.
[7] Kumplee, S., Havananda, T. and Saradhuldhat, P., 2015, Multiplications of 'MD2' pineapple by crown splitting technique, Agric. Sci. J. 46(Suppl. 3): 121-124. (in Thai)
[8] Ngomuo, M., Mneney, E. and Ndakidemi, P., 2014, The effect of bud splitting on suppression of apical dominance and inducing multiple buds development in banana shoots tip cultures of cv. ‘Yangambi’ (AAA) in Tanzania, Am. J. Exp. Agric. 4: 1853-1860.
[9] Israeli, Y., Lahav, E. and Reuveni, O., 1995, In vitro Culture of Bananas, pp. 147-178, Bananas and Plantains, World Crop Series, Springer, Dordrecht.
[10] Jarassamrit, N., 1994, Plant Hormones and Plant Growth Regulators, Sahamit Off Set, Bangkok, 124 p. (in Thai)
[11] Tongumpai, P., 1986, Plant Hormones, Kasetsart University, Kasetsart University, 196 p. (in Thai)
[12] Tudses, N., Phungbunhan, K. and Audta, A., 2019, Effects of BA and NAA on growth and development of Musa sapientum (ABB group) cv ‘Kluai Hin’ in vitro and effects of substrate on growth in vivo, King Mongkut’s Agric. J. 37(2): 262-273. (in Thai)
[13] Siriphanich, J., 2010, Postharvest Biology and Plant Senescence, Kasetsart University, Nakhon Pathom, 453 p. (in Thai)
[14] Wattanaphansuk, S., 2006, The Effects of Anti-Browning Agents and Modified Atmosphere Packaging on Shelf-Life of Fresh-Cut Lettuce, Department of Food Technology, Graduate School, Silpakorn University, Nakhon Pathom. (in Thai)
[15] Yensai, P. and Suraninpong, P., 2014, Effect of benzyladenine and thidiazuron on multiple shoot induction in ‘Kluai Chang’ in vitro, Khon Kaen Agric. J. 42(Suppl. 3): 157-161. (in Thai)
[16] Kawat, L., Nakhon, M., Suwanwong, S., Tantiwiwat, S. and Wongkantharakorn, N., 2013, Plant Physiology, Kasetsart University, Bangkok, 270 p. (in Thai)