Size and Cutting Pattern of Explant for Micropropagation of Musa (AA Group) ‘Kluai Khai Kasetsart 2’

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พรนัชชา คงพันธุ์
อารยา อาจเจริญ เทียนหอม
ทัศไนย จารุวัฒนพันธ์

Abstract

Effects of explant size and cutting pattern of Musa (AA group) ‘Kluai Khai Kasetsart 2’ in vitro culture were studied. Two sucker sizes were used: 1 (1 x 1 x 1 cm) and 1.5 (1.5 x 1.5 x 1.5) cm. Both sized suckers were not divided and divided in to 2 and 4 pieces then the explants were cultured on MS medium supplemented with 5 mg/L BA. Intact explant size 1.5 cm was used as a control. The experiment was arranged in a completely randomized design with 10 replications. The result showed that sucker size and cutting pattern affected plantlet survival percentages. Explants size 1.5 cm with 2- and 4-piece cut had survival rate at 60 and 50 %, respectively, which were higher than that of the control (35%), while explant size 1 cm with all cuts had 80% survival rate. The explants size 1 cm with 2- and 4-piece cuts and size 1.5 cm with 4-piece cut gave the highest new shoot numbers (72, 77 and 66 shoots, respectively). Subsequently, the size 1 cm explants were pinched at the terminal bud by scooping method. The pinched explants had higher regenerated shoots (29) than the non-pinched ones (12).

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วิทยาศาสตร์ชีวภาพ
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พรนัชชา คงพันธุ์, คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

อารยา อาจเจริญ เทียนหอม

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

ทัศไนย จารุวัฒนพันธ์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

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