Size and Cutting Pattern of Explant for Micropropagation of Musa (AA Group) ‘Kluai Khai Kasetsart 2’
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Abstract
Effects of explant size and cutting pattern of Musa (AA group) ‘Kluai Khai Kasetsart 2’ in vitro culture were studied. Two sucker sizes were used: 1 (1 x 1 x 1 cm) and 1.5 (1.5 x 1.5 x 1.5) cm. Both sized suckers were not divided and divided in to 2 and 4 pieces then the explants were cultured on MS medium supplemented with 5 mg/L BA. Intact explant size 1.5 cm was used as a control. The experiment was arranged in a completely randomized design with 10 replications. The result showed that sucker size and cutting pattern affected plantlet survival percentages. Explants size 1.5 cm with 2- and 4-piece cut had survival rate at 60 and 50 %, respectively, which were higher than that of the control (35%), while explant size 1 cm with all cuts had 80% survival rate. The explants size 1 cm with 2- and 4-piece cuts and size 1.5 cm with 4-piece cut gave the highest new shoot numbers (72, 77 and 66 shoots, respectively). Subsequently, the size 1 cm explants were pinched at the terminal bud by scooping method. The pinched explants had higher regenerated shoots (29) than the non-pinched ones (12).
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References
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