Evaluation of the Antioxidant Activities of Black Single Bulb Form of Elephant Garlic (Allium ampeloprasum var. ampeloprasum)

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ชุติมา แก้วพิบูลย์
ณวงศ์ บุนนาค

Abstract

Black garlic has high nutritional value, with different texture, smell and taste from fresh garlic, resulting in easier intake. The aim of this study was to evaluate the antioxidant activity of black single bulb form of elephant garlic (BSBG) by using DPPH, ABTS and FRAP methods, compared with fresh garlic (FG). By means of Folin-Ciocalteu colorimetric method, total phenolic content in BSBG was higher than that of FG (31.15±0.39 and 9.76±0.60 mg GAE/g extract, respectively). Likewise, by using DPPH and ABTS assays, BSBG showed more antioxidant activities than FG. The EC50 values for DPPH were 4.30±0.13 and 3.80±0.16 mg/mL, while the EC50 values for ABTS were 15.91±0.33 and 8.87±0.26 mg/mL, respectively. In addition, by FRAP assay, BSBG possessed higher ferric ion reducing antioxidant power than FG, with FRAP values of 443.05± 21.24 and 117.33±4.76 mM Fe2+/g extract, respectively. Therefore, BSBG is more effective antioxidant capacity compared to FG, and suitable to be a healthy food product.


 

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Section
วิทยาศาสตร์ชีวภาพ
Author Biographies

ชุติมา แก้วพิบูลย์

สาขาวิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตพัทลุง ตำบลบ้านพร้าว อำเภอป่าพะยอม จังหวัดพัทลุง 93210 

ณวงศ์ บุนนาค

สาขาวิชาวิทยาศาสตร์และคณิตศาสตร์พื้นฐาน คณะวิทยาศาสตร์ มหาวิทยาลัยทักษิณ วิทยาเขตสงขลา ตำบลเขารูปช้าง อำเภอเมือง จังหวัดสงขลา 90000

References

[1] Mikaili, P., Maadirad, S., Moloudizargari, M., Aghajanshakeri, S. and Sarahroodi, S., 2013, Therapeutic uses and pharmaco logical properties of garlic, shallot, and their biologically active compounds, Iran. J. Basic Med. Sci. 16: 1031-1048.
[2] Bayan, L., Koulivand, P.H. and Gorji, A., 2013, Garlic: A review of potential therapeutic effects, Avicenna J. Phytomed. 4: 1-14.
[3] Adaki, S., Adaki, R., Shah, K. and Karagir, A., 2014, Garlic: Review of literature, Ind. J. Cancer 51: 577-581.
[4] Choi, I.S., Cha, H.S. and Lee, Y.S., 2014, Physicochemical and antioxidant properties of black garlic, Molecules 19: 16811-16823.
[5] Kang, O.J., 2016, Physicochemical characteristics of black garlic after different thermal processing steps, Prev. Nutr. Food Sci. 21: 348-354.
[6] Kimura, S., Tung, Y.C., Pan, M.H., Su, N.W., Lai, Y.J. and Cheng, K.C., 2017, Black garlic: A critical review of its production, bioactivity, and application, J. Food Drug Anal. 25: 62-70.
[7] Kim, J.S., Kang, O.J. and Gweon, O.C., 2013, Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps, J. Func. Foods 5: 80-86.
[8] Yang, G.Q., Wang, D., Wang, Y.S., Wang, Y.Y. and Yang, K., 2013, Radiosensitization effect of black garlic extract on lung cancer cell line Lewis cells, Chin. J. Integr. Med. 33: 1093-1097.
[9] Ha, A.W., Ying, T. and Kim, W.K., 2015, The effects of black garlic (Allium sativum) extracts on lipid metabolism in rats fed a high fat diet, Nutr. Res. Pract. 9: 30-36.
[10] Tak, H.M., Kang, M.J., Kyoung, M.K., Kang, D., Han, S. and Shin, J.H., 2014, Anti-inflammatory activities of fermented black garlic, Korean J. Food Nutr. 43: 1527-1534.
[11] Jang, E.K., Seo, J.H. and Lee, S.P., 2008, Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) extract, Korean J. Food Sci. Technol. 40: 443-448.
[12] Toledano-Medina, M.A., Pérez-Aparicio, J., Moreno-Rojas, R. and Merinas-Amo, T., 2016, Evolution of some physicochemical and antioxidant properties of black garlic whole bulbs and peeled cloves Food Chem. 199: 135-139.
[13] Xu, G., Ye, X., Chen, J. and Liu, D., 2007, Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract, J. Agric. Food Chem. 55: 330-335.
[14] Sutharut, J. and Sudarat, J., 2012, Total anthocyanin content and antioxidant activity of germinated colored rice, Int Food Res. J. 19: 215-221.
[15] Prior, R.L., Wu, X. and Schaich, K., 2005, Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements, J. Agric. Food Chem. 53: 4290-4302.
[16] Floegel, A., Kim, D.O., Chung, S.J., Koo, S.I. and Chun, O.K., 2011, Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods, J. Food Comp. Anal. 24: 1043-1048.
[17] Jang, H.J., Lee, H.J., Yoon, D.K., Ji, D.S., Kim, J.H. and Lee, C.H., 2017, Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents, Food Sci. Biotechnol. 27: 219-225.
[18] Pietta, P.G., 2000, Flavonoids as antioxidants, J. Nat. Prod. 63: 1035-1042.