ชมพู่ทับทิมจันทร์เพิ่มกระบวนการส่งสัญญาณของอินซูลินในตับของหนูเบาหวาน
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Published:
Jan 5, 2020
Keywords:
Taaptimjann wax apple diabetes liver insulin signaling
Main Article Content
Abstract
The aim of this study was to investigate the effect of Taaptimjann wax apple [Syzygium samarangense (Blume) Merrill. & L.M. Perry var. Samarangense] on hepatic insulin signaling in the liver of male diabetic rats. Diabetic rats were induced in Sprague-Dawley rats using a single intraperitoneal (i.p.) injection of streptozotocin [STZ; 65 mg/kg body weight (b.w.)]. Then, wax apple at dose of 100 mg/kg b.w./day was orally administered to diabetic rats for a period of 4 weeks. At the end of experiment, blood glucose level, and lipid peroxidation were evaluated. Hepatic expression levels of insulin signaling proteins were also measured using Western blot analysis. The results clearly showed STZ induction provided an increase in blood glucose level and maintained hyperglycemia throughout this experiment. Also, the expression levels of insulin signaling-related proteins; insulin receptor-β (InR-β), phospho-insulin receptor substrate 1 [p-IRS1 (Ser307)], and phospho-JNK [p-JNK (Thr183/Tyr185)] were upregulated. Malondialdehyde (MDA) level was also noted in liver of STZ-induced diabetic rats. However, treatment with wax apple at a dose of 100 mg/kg b.w. to STZ-induced diabetic rats produced a significant decrease in blood glucose level, which correlated with downregulation of InR-β, p-IRS1 (Ser307), and p-JNK (Thr183/Tyr185) proteins in the liver. Wax apple also diminished the level of MDA in liver of diabetic rats. These findings therefore suggest that wax apple could improve hepatic insulin signaling pathway in STZ-induced diabetic rats, which is associated with decreased oxidative stress.
Article Details
Section
Medical Sciences
References
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[28] Goyal, S.N., Reddy, N.M., Patil, K.R., Nakhate, K.T., Ojha, S., Patil, C.R. and Agrawal, Y.O., 2016, Challenges and issues with streptozotocin-induced diabetes: A clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics, Chem. Biol. Interact. 244: 49-63.
[29] Lenzen, S., 2008, The mechanisms of alloxan- and streptozotocin-induced diabetes, Diabetologia 51: 216-226.
[30] Boucher, J., Kleinridders, A. and Kahn, C.R., 2014, Insulin receptor signaling in normal and insulin-resistant states, Cold Spring Harb. Perspect. Biol. 6(1): a009191.
[31] Rains, J.L. and Jain, S.K., 2011, Oxidative stress, insulin signaling, and diabetes, Free Rad. Biol. Med. 50: 567-575.
[32] Boucher, J., Kleinridders, A. and Kahn, C.R., 2014, Insulin receptor signaling in normal and insulin-resistant states, Cold Spring Harb Perspect Biol. 6(1): a009191.
[33] Zhou, J., Xu, G., Bai, Z., Li, K., Yan, J., Li, F., Ma, S., Xu, H. and Huang, K., 2015, Selenite exacerbates hepatic insulin resistance in mouse model of type 2 diabetes through oxidative stress-mediated JNK pathway, Toxicol. Appl. Pharm. 289: 409-418.
[34] Al-Attar, A.M. and Alsalmi, F.A., 2017, Effect of Olea europaea leaves extract on streptozotocin induced diabetes in male albino rats, Saudi. J. Biol. Sci. 26: 118-128.
[35] Jayachandran, M., Zhang, T., Ganesan, K., Xu, B. and Chung, S.S.M., 2018, Isoquercetin ameliorates hyperglycemia and regulates key enzymes of glucose metabolism via insulin signaling pathway in streptozotocin-induced diabetic rats, Eur. J. Pharm. 829: 112-120.
[36] Vinayagam, R., Jayachandran, M., Chung, S.S.M. and Xu, B., 2018, Guava leaf inhibits hepatic gluconeogenesis and increases glycogen synthesis via AMPK/ACC signaling pathways in streptozotocin-induced diabetic rats, Biomed Pharm. 103: 1012-1017.
[37] Wei, Y., Gao, J., Qin, L., Xu, Y., Wang, D., Shi, H., Xu, T. and Liu, T., 2017, Tanshinone I alleviates insulin resistance in type 2 diabetes mellitus rats through IRS-1 pathway, Biomed Pharm. 93: 352-358.
[38] Kadowaki, T., Kasuga, M., Akanuma, Y., Ezaki, O. and Takaku, F., 1984, Decreased autophosphorylation of the insulin receptor-kinase in streptozotocin-diabetic rats, J. Biol. Chem. 259: 14208-14216.
[39] Tozzo, E. and Desbuquois, B., 1992, Effects of STZ-induced diabetes and fasting on insulin receptor mRNA expression and insulin receptor gene transcription in rat liver, Diabetes 41: 1609.
[40] Vinayagam, R. and Xu, B., 2015, Antidiabetic properties of dietary flavonoids: A cellular mechanism review, Nutr. Metab. (Lond). 12: 60.
[41] Yang, L., Wang, Z., Jiang, L., Sun, W., Fan, Q. and Liu, T., 2017, Total flavonoids extracted from Oxytropis falcata Bunge improve insulin resistance through regulation on the IKKβ/NF-κB inflammatory pathway, Evid. Based Complementary Altern Med. 2017: 2405124.
[42] M., S.S. and C., D.N., 2017, Influence of quercetin, naringenin and berberine on glucose transporters and insulin signalling molecules in brain of streptozotocin-induced diabetic rats, Biomed Pharma. 94: 605-611.
[43] Zhang, Z.F., Lu, J., Zheng, Y.L., Wu, D.M., Hu, B., Shan, Q., Cheng, W., Li, M.Q. and Sun, Y.Y., 2013, Purple sweet potato color attenuates hepatic insulin resistance via blocking oxidative stress and endoplasmic reticulum stress in high-fat-diet-treated mice, J. Nutr. Biochem. 24: 1008-1018.
[44] Kim, Y.J., Kim, H.C., Ko, H., Amor, E.C., Lee, J.W. and Yang, H.O., 2012, Inhibitory effects of aurentiacin from Syzygium samarangense on lipopolysaccharide-induced inflammatory response in mouse macrophages, Food Chem. Toxicol. 50: 1027-1035.
[45] Shen, S.C., Chang, W.C. and Chang, C.L., 2013, An extract from wax apple [Syzygium samarangense (Blume) Merrill & Perry] effects glycogenesis and glycolysis pathways in tumor necrosis factor-alpha-treated FL83B mouse hepatocytes, Nutrients 5: 455-467.
[2] American Diabetes Association, 2009, Diagnosis and classification of diabetes mellitus, Diab. Care 32: 62-67.
[3] Forbes, J.M. and Cooper, M.E., 2013, Mechanisms of diabetic complications, Physiol. Rev. 93: 137-188.
[4] Wilcox, G., 2005, Insulin and insulin resistance, Clin. Biochem. Rev. 26: 19-39.
[5] Postic, C., Dentin, R. and Girard, J., 2004, Role of the liver in the control of carbohydrate and lipid homeostasis, Diab. Metab. 30: 398-408.
[6] Rui, L., 2014, Energy metabolism in the liver, Compr. Physiol. 4: 177-197.
[7] Edgerton, D.S., Kraft, G., Smith, M., Farmer, B., Williams, P.E., Coate, K.C., Printz, R.L., O’Brien, R.M. and Cherrington, A.D., 2017, Insulin’s direct hepatic effect explains the inhibition of glucose production caused by insulin secretion, JCI Insight 2: 91863.
[8] Parveen, K., Khan, M.R., Mujeeb, M. and Siddiqui, W.A., 2010, Protective effects of Pycnogenol® on hyperglycemia-induced oxidative damage in the liver of type 2 diabetic rats, Chem. Biol. Interact. 186: 219-227.
[9] Trauner, M., Arrese, M. and Wagner, M., 2010, Fatty liver and lipotoxicity, Biochim. Biophys. Acta 1801: 299-310.
[10] Meshkani, R. and Adeli, K., 2009, Hepatic insulin resistance, metabolic syndrome and cardiovascular disease, Clin. Biochem.
42: 1331-1346.
[11] Adiels, M., Taskinen, M.R. and Boren, J., 2008, Fatty liver, insulin resistance, and dyslipidemia, Curr. Diab. Rep. 8: 60-64.
[12] King, G.L. and Loeken, M.R., 2004, Hyperglycemia-induced oxidative stress in diabetic complications, Histochem. Cell Biol. 122: 333-338.
[13] Tomas, E., Lin, Y.S., Dagher, Z., Saha, A., Luo, Z., Ido, Y. and Ruderman, N.B., 2002, Hyperglycemia and insulin resistance: Possible mechanisms, Ann. N Y Acad. Sci. 967: 43-51.
[14] Buse, M.G., 2006, Hexosamines, insulin resistance, and the complications of diabetes: current status, Am. J. Physiol. Endocrinol. Metab. 290: 1-8.
[15] McClain, D.A. and Crook, E.D., 1996, Hexosamines and insulin resistance, Diabetes 45: 1003-1009.
[16] Shulman, G.I., 2000, Cellular mechanisms of insulin resistance, J. Clin. Invest. 106: 171-176.
[17] Aguirre, V., Uchida, T., Yenush, L., Davis, R. and Whitei, M.F., 2000, The c-Jun NH2-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser307, J. Biol. Chem. 275: 9047-9054.
[18] Yang, R. and Trevillyan, J.M., 2008, c-Jun N-terminal kinase pathways in diabetes, Int. J. Biochem. Cell Biol. 40: 2702-2706.
[19] Khandaker, M.M. and Boyce, A.N., 2016, Growth, distribution and physiochemical properties of wax apple (Syzygium samarangense): A review, Aust. J. Crop Sci. 10: 1640-1648.
[20] Kuo, Y.C., Yang, L.M. and Lin, L.C., 2004, Isolation and immunomodulatory effect of flavonoids from Syzygium samara gense, Plant. Med. 70: 1237-1239.
[21] Nair, A.G.R., Krishnan, S., Ravikrishna, C. and Madhusudanan, K.P., 1999, New and rare flavonol glycosides from leaves of Syzygium samarangense, Fitoterapia 70: 148-151.
[22] Resurreccion-Magno, M.H., Villasenor, I.M., Harada, N. and Monde, K., 2005, Anti hyperglycaemic flavonoids from Syzygium samarangense (Blume) Merr. and Perry, Phytother. Res. 19: 246-251.
[23] Khandaker, M.M., Boyce, A.N., Osman, N. and Hossain, A.S., 2012, Physiochemical and phytochemical properties of wax apple (Syzygium samarangense [Blume] Merrill & L. M. Perry var. Jambu Madu) as affected by growth regulator application, Sci. World J. 2012: 728613.
[24] Srivastava, R., Shaw, A.K. and Kulshreshtha, D.K., 1995, Triterpenoids and chalcone from Syzygium samarangense, Phyto chemistry 38: 687-689.
[25] Shen, S.C. and Chang, W.C., 2013, Hypotriglyceridemic and hypoglycemic effects of vescalagin from Pink wax apple [Syzygium samarangense (Blume) Merrill and Perry cv. Pink] in high-fructose diet-induced diabetic rats, Food Chem. 136: 858-863.
[26] Shen, S.C., Chang, W.C. and Chang, C.L., 2012, Fraction from Wax Apple [Syzygium samarangense (Blume) Merrill and Perry] Fruit extract ameliorates insulin resistance via modulating insulin signaling and inflammation pathway in tumor necrosis factor α-treated FL83B mouse hepato cytes, Int. J. Mol. Sci. 13: 8562-8577.
[27] Khamchan, A., Paseephol, T. and Hanchang, W., 2018, Protective effect of wax apple [Syzygium samarangense (Blume) Merr. & L.M. Perry] against streptozotocin-induced pancreatic β-cell damage in diabetic rats, Biomed. Pharm. 108: 634-645.
[28] Goyal, S.N., Reddy, N.M., Patil, K.R., Nakhate, K.T., Ojha, S., Patil, C.R. and Agrawal, Y.O., 2016, Challenges and issues with streptozotocin-induced diabetes: A clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics, Chem. Biol. Interact. 244: 49-63.
[29] Lenzen, S., 2008, The mechanisms of alloxan- and streptozotocin-induced diabetes, Diabetologia 51: 216-226.
[30] Boucher, J., Kleinridders, A. and Kahn, C.R., 2014, Insulin receptor signaling in normal and insulin-resistant states, Cold Spring Harb. Perspect. Biol. 6(1): a009191.
[31] Rains, J.L. and Jain, S.K., 2011, Oxidative stress, insulin signaling, and diabetes, Free Rad. Biol. Med. 50: 567-575.
[32] Boucher, J., Kleinridders, A. and Kahn, C.R., 2014, Insulin receptor signaling in normal and insulin-resistant states, Cold Spring Harb Perspect Biol. 6(1): a009191.
[33] Zhou, J., Xu, G., Bai, Z., Li, K., Yan, J., Li, F., Ma, S., Xu, H. and Huang, K., 2015, Selenite exacerbates hepatic insulin resistance in mouse model of type 2 diabetes through oxidative stress-mediated JNK pathway, Toxicol. Appl. Pharm. 289: 409-418.
[34] Al-Attar, A.M. and Alsalmi, F.A., 2017, Effect of Olea europaea leaves extract on streptozotocin induced diabetes in male albino rats, Saudi. J. Biol. Sci. 26: 118-128.
[35] Jayachandran, M., Zhang, T., Ganesan, K., Xu, B. and Chung, S.S.M., 2018, Isoquercetin ameliorates hyperglycemia and regulates key enzymes of glucose metabolism via insulin signaling pathway in streptozotocin-induced diabetic rats, Eur. J. Pharm. 829: 112-120.
[36] Vinayagam, R., Jayachandran, M., Chung, S.S.M. and Xu, B., 2018, Guava leaf inhibits hepatic gluconeogenesis and increases glycogen synthesis via AMPK/ACC signaling pathways in streptozotocin-induced diabetic rats, Biomed Pharm. 103: 1012-1017.
[37] Wei, Y., Gao, J., Qin, L., Xu, Y., Wang, D., Shi, H., Xu, T. and Liu, T., 2017, Tanshinone I alleviates insulin resistance in type 2 diabetes mellitus rats through IRS-1 pathway, Biomed Pharm. 93: 352-358.
[38] Kadowaki, T., Kasuga, M., Akanuma, Y., Ezaki, O. and Takaku, F., 1984, Decreased autophosphorylation of the insulin receptor-kinase in streptozotocin-diabetic rats, J. Biol. Chem. 259: 14208-14216.
[39] Tozzo, E. and Desbuquois, B., 1992, Effects of STZ-induced diabetes and fasting on insulin receptor mRNA expression and insulin receptor gene transcription in rat liver, Diabetes 41: 1609.
[40] Vinayagam, R. and Xu, B., 2015, Antidiabetic properties of dietary flavonoids: A cellular mechanism review, Nutr. Metab. (Lond). 12: 60.
[41] Yang, L., Wang, Z., Jiang, L., Sun, W., Fan, Q. and Liu, T., 2017, Total flavonoids extracted from Oxytropis falcata Bunge improve insulin resistance through regulation on the IKKβ/NF-κB inflammatory pathway, Evid. Based Complementary Altern Med. 2017: 2405124.
[42] M., S.S. and C., D.N., 2017, Influence of quercetin, naringenin and berberine on glucose transporters and insulin signalling molecules in brain of streptozotocin-induced diabetic rats, Biomed Pharma. 94: 605-611.
[43] Zhang, Z.F., Lu, J., Zheng, Y.L., Wu, D.M., Hu, B., Shan, Q., Cheng, W., Li, M.Q. and Sun, Y.Y., 2013, Purple sweet potato color attenuates hepatic insulin resistance via blocking oxidative stress and endoplasmic reticulum stress in high-fat-diet-treated mice, J. Nutr. Biochem. 24: 1008-1018.
[44] Kim, Y.J., Kim, H.C., Ko, H., Amor, E.C., Lee, J.W. and Yang, H.O., 2012, Inhibitory effects of aurentiacin from Syzygium samarangense on lipopolysaccharide-induced inflammatory response in mouse macrophages, Food Chem. Toxicol. 50: 1027-1035.
[45] Shen, S.C., Chang, W.C. and Chang, C.L., 2013, An extract from wax apple [Syzygium samarangense (Blume) Merrill & Perry] effects glycogenesis and glycolysis pathways in tumor necrosis factor-alpha-treated FL83B mouse hepatocytes, Nutrients 5: 455-467.