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Lung cancer has been a disease with high fatality rate due to the high metastatic rate. One of the most essential processes of metastasis is the ability of cancer cells to undergo the epithelial to mesenchymal transition (EMT) which allows cancer cells to resist the programmed cell death in a detached condition and to migrate into the surrounding organ tissue. Andrographolide has been a promising naturally derived compound for cancer therapy due to several cytotoxic effects in various cancer cells. Therefore, this study has demonstrated that andrographolide inhibits cell proliferation and metastasis process in A549 human lung cancer cells. Cytotoxicity of andrographolide on A549 and Vero cells using MTT assay showed that andrographolide decreased the viability of A549 human lung cancer cells with IC50 value at 5.51 µg/mL, whereas it performed non-cytotoxic against Vero normal cell lines. Furthermore, andrographolide decreased the migration process. As the results, andrographolide is found to be a potential compound for studying its mechanisms to further develop as cancer therapeutic drug.
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