การขยายพันธุ์ด้วยการเพาะเลี้ยงเนื้อเยื่อของกระเทียมน้ำ (Isoetes coromandelina L.f.) : ไลโคไฟต์หายากของประเทศไทย

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ศรัณย์พร ล่องชูผล
อารยา อาจเจริญ เทียนหอม
ทัศไนย จารุวัฒนพันธ์

Abstract

A micropropagation protocol was developed for Isoetes coromandelina, a species threatened due to habitat destruction in Thailand. Corm-like explants were surface sterilized with Clorox®, mercuric chloride (HgCl2) and antibiotic drug (tetracycline). The result showed that using 250 ppm tetracycline for 20 minutes followed by 10 % (v/v) Clorox® for 15 minutes and 0.2 % (w/v) HgCl2 for 10 minutes resulted in the highest survival percentage (55.56 %). The corm-like explants were cultured in ½ MS for 4 weeks and cultured on ½ MS containing with 0.5, 1, 1.5 and 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) to induce callus. The result showed that the treatment group using 0.5 mg/L 2,4-D had the highest survival percentage (75 %) and callus was induced. The cleaned corm-like explants were cultured on ½ MS containing 0, 0.5 and 1 mg/L BA, and 0, 0.5 and 1 mg/L NAA for 8 weeks. The result showed that the explants cultured on medium without BA had the longest leaves while the highest number of roots occurred on medium supplemented with 1.0 mg/L NAA.

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Section
วิทยาศาสตร์ชีวภาพ
Author Biographies

ศรัณย์พร ล่องชูผล

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

อารยา อาจเจริญ เทียนหอม

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

ทัศไนย จารุวัฒนพันธ์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

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