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A micropropagation protocol was developed for Isoetes coromandelina, a species threatened due to habitat destruction in Thailand. Corm-like explants were surface sterilized with Clorox®, mercuric chloride (HgCl2) and antibiotic drug (tetracycline). The result showed that using 250 ppm tetracycline for 20 minutes followed by 10 % (v/v) Clorox® for 15 minutes and 0.2 % (w/v) HgCl2 for 10 minutes resulted in the highest survival percentage (55.56 %). The corm-like explants were cultured in ½ MS for 4 weeks and cultured on ½ MS containing with 0.5, 1, 1.5 and 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) to induce callus. The result showed that the treatment group using 0.5 mg/L 2,4-D had the highest survival percentage (75 %) and callus was induced. The cleaned corm-like explants were cultured on ½ MS containing 0, 0.5 and 1 mg/L BA, and 0, 0.5 and 1 mg/L NAA for 8 weeks. The result showed that the explants cultured on medium without BA had the longest leaves while the highest number of roots occurred on medium supplemented with 1.0 mg/L NAA.
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