Mycobacterium tuberculosis Complex Detection in Formalin-fixed, Paraffin-embedded Tissue Using abTESTM MTB qPCR KIT in Comparison with Conventional Polymerase Chain Reaction
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Abstract
Tuberculosis (TB) is the crucial cause of death from infectious diseases worldwide. The causative agents of TB are a group of closely related bacteria known as the Mycobacterium tuberculosis complex (MTBC). Accurate and rapid diagnosis by PCR method is helpful for the successful treatment of tuberculosis. The aim of this study was to compare the efficiency between a more rapid method by a commercial kit (abTESTM MTB qPCR kit) and a cheaper but less rapid method by conventional polymerase chain reaction (C-PCR) for the detection of M. tuberculosis complex in FFPE tissue. Six hundred and forty formalin-fixed paraffin-embedded (FFPE) tissues that showed histomorphology suspected of tuberculous infection were collected from the Institute of Pathology, Department of Medical Services. DNA was isolated from the FFPE tissue and two methods of PCR were performed by using the abTESTM MTB qPCR kit and C-PCR for the detection of M. tuberculosis complex. The results showed that C-PCR was more sensitive for detection of M. tuberculosis complex than abTESTM MTB qPCR kit. There were 199 samples (30 %) positive for TB-DNA by C-PCR and 192 samples (28 %) positive for TB-DNA by abTESTM MTB qPCR kit. The comparison between these two methods by kappa analysis showed a very good agreement (K = 0.959, 95 % CI 0.936-0.983). In conclusion, C-PCR is more effective for the detection of TB DNA in FFPE tissue. This less costly method (even taking longer time) is an alternative method for the detection of MTBC infection in FFPE tissue.
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References
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