ฤทธิ์ทางชีวภาพของสารเบอร์บีรีนที่แยกจากเถาแห้ม
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Published:
Feb 21, 2020
Keywords:
Coscinium fenestratum berberine biological activity
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Abstract
Berberine from crude methanol extract of Coscinium fenestratum stem was purified by column chromatography using methanol : acetic acid : water solvent system (13 : 0.7 : 1). The berberine structure was elucidated by spectroscopic techniques. After that, the biological activities of berberine were investigated, including α-glucosidase and amylase inhibitory activities, antioxidant activities, and cytotoxicity against human lung cancer cell line A549. We found that berberine showed effective cytotoxicity against A549 cell line with an IC50 value of 20.33±3.51 μg/mL. Therefore, this study provided important information for a promising lead compound for of anticancer drug development.
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Biological Sciences
References
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[15] Li, X., Zhao, S.J., Shi, H.L., Qiu, S.P., Xie, J.Q., Wu, H., Zhang, B.B., Wang, Z.T., Yuan, J.Y. and Wu, X.J., 2014, Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells, Oncol. Rep. 32: 2777-2788.
[16] Li, H.L., Wu, H., Zhang, B.B., Shi, H.L. and Wu, X.J., 2016, MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo, Mol. Med. Rep. 14: 1430-1438.
[17] Xi, S., Chuang, K., Fang, K., Lee, Y., Chung, J. and Chuang, Y., 2014, Effect of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549, J. Tradit. Chin. Med. 34: 302-308.
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[3] Yin, J., Ye, J. and Jia, W., 2012, Effects and mechanisms of berberine in diabetes treatment, Acta Pharm. Sin. B 2: 327-334.
[4] Pang, B., Zhao, L.H., Zhou, Q., Zhao, T.Y., Wang, H., Gu, C.J. and Tong, X.L., 2015, Application of berberine on treating type 2 diabetes mellitus, Int. J. Endocrinol. 2015: 905749-905749.
[5] Lin, J.G., Chung, J.G., Wu, L.T., Chen, G.W., Chang, H.L. and Wang, T.F., 1999, Effects of berberine on arylamine N-acetyl transferase activity in human colon tumor cells, Am. J. Chin. Med. 27: 265-275.
[6] Fukuda, K., Hibiya, Y., Mutoh, M., Koshiji, M., Akao, S. and Fujiwara, H., 1999, Inhibition by berberine of cyclooxyge nase-2 transcriptional activity in human colon cancer cells, J. Ethnopharm. 66: 227-233.
[7] Rahman, A. and William, P.L.Q., 1988, Natural Products Chemistry III, Springer, Heidelberg, 359 p.
[8] Kaewpiboon, C., Lirdprapamongkol, K., Srisomsap, C., Winayanuwattikun, P., Yongvanich, T., Puwaprisirisan, P., Svasti, J. and Assavalapsakul, W., 2012, Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants, BMC Comp. Altern. Med. 12: 217.
[9] Kaewpiboon, C., Boonnak, N., Kaowinn, S. and Chung, Y.H., 2018, Formoxanthone C, isolated from Cratoxylum formosum ssp. pruniflorum, reverses anticancer drug resistance by inducing both apoptosis and autophagy in human A549 lung cancer cells, Bioorg. Med. Chem. Lett. 28: 820-825.
[10] Jhong, C.H., Riyaphan, J., Lin, S.H., Chia, Y.C. and Weng, C.F., 2015, Screening alpha-glucosidase and alpha-amylase inhibitors from natural compounds by molecular docking in silico, Biofactors 41: 242-251.
[11] Liu, L., Deng, Y., Yu, S., Lu, S., Xie, L. and Liu, X., 2008, Berberine attenuates intestinal disaccharidases in streptozotocin-induced diabetic rats, Pharmazie 63: 384-388.
[12] Pongkittiphan, V., Chavasiri, W. and Supabphol, R., 2015, Antioxidant effect of berberine and its phenolic derivatives against human fibrosarcoma cells, Asian Pac. J. Cancer Prev. 16: 5371-5376.
[13] Valgimigli, L., Amorati, R., Fumo, M.G., DiLabio, G.A., Pedulli, G.F., Ingold, K.U. and Pratt, D.A., 2008, The unusual reaction of semiquinone radicals with molecular oxygen, J. Org. Chem. 73: 1830-1841.
[14] Li, J., Liu, F., Jiang, S., Liu, J., Chen, X., Zhang, S. and Zhao, H., 2018, Berberine hydrochloride inhibits cell proliferation and promotes apoptosis of non-small cell lung cancer via the suppression of the MMP2 and Bcl-2/Bax signaling pathways, Oncol. Lett. 15: 7409-7414.
[15] Li, X., Zhao, S.J., Shi, H.L., Qiu, S.P., Xie, J.Q., Wu, H., Zhang, B.B., Wang, Z.T., Yuan, J.Y. and Wu, X.J., 2014, Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells, Oncol. Rep. 32: 2777-2788.
[16] Li, H.L., Wu, H., Zhang, B.B., Shi, H.L. and Wu, X.J., 2016, MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo, Mol. Med. Rep. 14: 1430-1438.
[17] Xi, S., Chuang, K., Fang, K., Lee, Y., Chung, J. and Chuang, Y., 2014, Effect of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549, J. Tradit. Chin. Med. 34: 302-308.