ฤทธิ์ทางชีวภาพของสารเบอร์บีรีนที่แยกจากเถาแห้ม
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ก.พ. 21, 2020
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แห้ม เบอร์บีรีน ฤทธิ์ทางชีวภาพ
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บทคัดย่อ
การแยกสารเบอร์บีรีนจากสารสกัดหยาบเมทานอลของเถาแห้มให้บริสุทธิ์ด้วยเทคนิคคอลัมน์โครมาโทกราฟี ในระบบตัวทำละลายผสมเมทานอล : กรดอะซิติก : น้ำ (13 : 0.7 : 1) แล้วพิสูจน์เอกลักษณ์ของสารโดยใช้เทคนิคสเปกโตรสโคปี จากนั้นทดสอบฤทธิ์ทางชีวภาพของสารเบอร์บีรีน ได้แก่ ฤทธิ์ยับยั้งการทำงานของแอลฟากลูโคซิเดส และอะไมเลส ฤทธิ์ต้านอนุมูลอิสระ และทดสอบความเป็นพิษต่อเซลล์มะเร็งปอดชนิด A549 พบว่าสารเบอร์บีรีนมีประสิทธิภาพในการยับยั้งการเจริญของเซลล์ A549 ได้ดีที่สุด มีค่า IC50 20.33±3.51 ไมโครกรัมต่อมิลลิลิตร ดังนั้นการศึกษานี้จึงให้ข้อมูลที่สำคัญของสารต้นแบบเพื่อการพัฒนาเป็นยารักษาโรคมะเร็ง
Article Details
ประเภทบทความ
Biological Sciences
เอกสารอ้างอิง
[1] Singburaudom, N., 2015, The alkaloid Berberine isolated from Coscinium fenes tratum is an inhibitor of phytopathogenic fungi, J. Biopest. 8: 28-36.
[2] Singh, T., Vaid, M., Katiyar, N., Sharma, S. and Katiyar, S.K., 2011, Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors, Carcinogenesis 32: 86-92.
[3] Yin, J., Ye, J. and Jia, W., 2012, Effects and mechanisms of berberine in diabetes treatment, Acta Pharm. Sin. B 2: 327-334.
[4] Pang, B., Zhao, L.H., Zhou, Q., Zhao, T.Y., Wang, H., Gu, C.J. and Tong, X.L., 2015, Application of berberine on treating type 2 diabetes mellitus, Int. J. Endocrinol. 2015: 905749-905749.
[5] Lin, J.G., Chung, J.G., Wu, L.T., Chen, G.W., Chang, H.L. and Wang, T.F., 1999, Effects of berberine on arylamine N-acetyl transferase activity in human colon tumor cells, Am. J. Chin. Med. 27: 265-275.
[6] Fukuda, K., Hibiya, Y., Mutoh, M., Koshiji, M., Akao, S. and Fujiwara, H., 1999, Inhibition by berberine of cyclooxyge nase-2 transcriptional activity in human colon cancer cells, J. Ethnopharm. 66: 227-233.
[7] Rahman, A. and William, P.L.Q., 1988, Natural Products Chemistry III, Springer, Heidelberg, 359 p.
[8] Kaewpiboon, C., Lirdprapamongkol, K., Srisomsap, C., Winayanuwattikun, P., Yongvanich, T., Puwaprisirisan, P., Svasti, J. and Assavalapsakul, W., 2012, Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants, BMC Comp. Altern. Med. 12: 217.
[9] Kaewpiboon, C., Boonnak, N., Kaowinn, S. and Chung, Y.H., 2018, Formoxanthone C, isolated from Cratoxylum formosum ssp. pruniflorum, reverses anticancer drug resistance by inducing both apoptosis and autophagy in human A549 lung cancer cells, Bioorg. Med. Chem. Lett. 28: 820-825.
[10] Jhong, C.H., Riyaphan, J., Lin, S.H., Chia, Y.C. and Weng, C.F., 2015, Screening alpha-glucosidase and alpha-amylase inhibitors from natural compounds by molecular docking in silico, Biofactors 41: 242-251.
[11] Liu, L., Deng, Y., Yu, S., Lu, S., Xie, L. and Liu, X., 2008, Berberine attenuates intestinal disaccharidases in streptozotocin-induced diabetic rats, Pharmazie 63: 384-388.
[12] Pongkittiphan, V., Chavasiri, W. and Supabphol, R., 2015, Antioxidant effect of berberine and its phenolic derivatives against human fibrosarcoma cells, Asian Pac. J. Cancer Prev. 16: 5371-5376.
[13] Valgimigli, L., Amorati, R., Fumo, M.G., DiLabio, G.A., Pedulli, G.F., Ingold, K.U. and Pratt, D.A., 2008, The unusual reaction of semiquinone radicals with molecular oxygen, J. Org. Chem. 73: 1830-1841.
[14] Li, J., Liu, F., Jiang, S., Liu, J., Chen, X., Zhang, S. and Zhao, H., 2018, Berberine hydrochloride inhibits cell proliferation and promotes apoptosis of non-small cell lung cancer via the suppression of the MMP2 and Bcl-2/Bax signaling pathways, Oncol. Lett. 15: 7409-7414.
[15] Li, X., Zhao, S.J., Shi, H.L., Qiu, S.P., Xie, J.Q., Wu, H., Zhang, B.B., Wang, Z.T., Yuan, J.Y. and Wu, X.J., 2014, Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells, Oncol. Rep. 32: 2777-2788.
[16] Li, H.L., Wu, H., Zhang, B.B., Shi, H.L. and Wu, X.J., 2016, MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo, Mol. Med. Rep. 14: 1430-1438.
[17] Xi, S., Chuang, K., Fang, K., Lee, Y., Chung, J. and Chuang, Y., 2014, Effect of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549, J. Tradit. Chin. Med. 34: 302-308.
[2] Singh, T., Vaid, M., Katiyar, N., Sharma, S. and Katiyar, S.K., 2011, Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors, Carcinogenesis 32: 86-92.
[3] Yin, J., Ye, J. and Jia, W., 2012, Effects and mechanisms of berberine in diabetes treatment, Acta Pharm. Sin. B 2: 327-334.
[4] Pang, B., Zhao, L.H., Zhou, Q., Zhao, T.Y., Wang, H., Gu, C.J. and Tong, X.L., 2015, Application of berberine on treating type 2 diabetes mellitus, Int. J. Endocrinol. 2015: 905749-905749.
[5] Lin, J.G., Chung, J.G., Wu, L.T., Chen, G.W., Chang, H.L. and Wang, T.F., 1999, Effects of berberine on arylamine N-acetyl transferase activity in human colon tumor cells, Am. J. Chin. Med. 27: 265-275.
[6] Fukuda, K., Hibiya, Y., Mutoh, M., Koshiji, M., Akao, S. and Fujiwara, H., 1999, Inhibition by berberine of cyclooxyge nase-2 transcriptional activity in human colon cancer cells, J. Ethnopharm. 66: 227-233.
[7] Rahman, A. and William, P.L.Q., 1988, Natural Products Chemistry III, Springer, Heidelberg, 359 p.
[8] Kaewpiboon, C., Lirdprapamongkol, K., Srisomsap, C., Winayanuwattikun, P., Yongvanich, T., Puwaprisirisan, P., Svasti, J. and Assavalapsakul, W., 2012, Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants, BMC Comp. Altern. Med. 12: 217.
[9] Kaewpiboon, C., Boonnak, N., Kaowinn, S. and Chung, Y.H., 2018, Formoxanthone C, isolated from Cratoxylum formosum ssp. pruniflorum, reverses anticancer drug resistance by inducing both apoptosis and autophagy in human A549 lung cancer cells, Bioorg. Med. Chem. Lett. 28: 820-825.
[10] Jhong, C.H., Riyaphan, J., Lin, S.H., Chia, Y.C. and Weng, C.F., 2015, Screening alpha-glucosidase and alpha-amylase inhibitors from natural compounds by molecular docking in silico, Biofactors 41: 242-251.
[11] Liu, L., Deng, Y., Yu, S., Lu, S., Xie, L. and Liu, X., 2008, Berberine attenuates intestinal disaccharidases in streptozotocin-induced diabetic rats, Pharmazie 63: 384-388.
[12] Pongkittiphan, V., Chavasiri, W. and Supabphol, R., 2015, Antioxidant effect of berberine and its phenolic derivatives against human fibrosarcoma cells, Asian Pac. J. Cancer Prev. 16: 5371-5376.
[13] Valgimigli, L., Amorati, R., Fumo, M.G., DiLabio, G.A., Pedulli, G.F., Ingold, K.U. and Pratt, D.A., 2008, The unusual reaction of semiquinone radicals with molecular oxygen, J. Org. Chem. 73: 1830-1841.
[14] Li, J., Liu, F., Jiang, S., Liu, J., Chen, X., Zhang, S. and Zhao, H., 2018, Berberine hydrochloride inhibits cell proliferation and promotes apoptosis of non-small cell lung cancer via the suppression of the MMP2 and Bcl-2/Bax signaling pathways, Oncol. Lett. 15: 7409-7414.
[15] Li, X., Zhao, S.J., Shi, H.L., Qiu, S.P., Xie, J.Q., Wu, H., Zhang, B.B., Wang, Z.T., Yuan, J.Y. and Wu, X.J., 2014, Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells, Oncol. Rep. 32: 2777-2788.
[16] Li, H.L., Wu, H., Zhang, B.B., Shi, H.L. and Wu, X.J., 2016, MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo, Mol. Med. Rep. 14: 1430-1438.
[17] Xi, S., Chuang, K., Fang, K., Lee, Y., Chung, J. and Chuang, Y., 2014, Effect of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549, J. Tradit. Chin. Med. 34: 302-308.
