Development of TaqMan Genotyping for Detection of Single Nucleotide Polymorphisms Associated with Latex Yield in Rubber Tree (Hevea brasiliensis)
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Abstract
The rubber tree is one of the leading commercial crops in Thailand. Phenotypic selection of hybrids performing high latex yield is time-consuming and low precision. Based on previous research showing single nucleotide polymorphisms (SNPs) associated with latex yield, this research aims to develop the TaqMan genotyping technique that can efficiently detect the SNPs associated with the rubber latex yield in the dry season and the wet season of Amazonian accessions. SNP genotyping of SNP6672 marker showed average latex yield in the dry season of heterozygous A/G that decreased 0.66 and 0.72 gram/tree/tapping average latex yield in the dry season of homozygous A/A and G/G. The heterozygous C/T of SNP14857 marker showed increasing in the average latex yield 1.37 gram/tree/tapping in the dry season and 3.0 gram/tree/tapping in the wet season, compared to the homozygous T/T. The TaqMan genotyping in this study would facilitate marker-assisted selection (MAS) of latex yield in large breeding populations with precision and shorter time.
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References
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