DNA Markers Assisted Type Determination of Cannabis sp.

Abstract

Cannabis sp. has the main active ingredient in the cannabinoids, i.e. tetrahydrocannabinol (THC) and cannabidiol (CBD). It was grouped by the content of THC and CBD. This research used molecular techniques to classify group of the Thai stick “Phuphan” as type 2 because the genes that synthesize both THC and CBD were found. Then the leaves explant was used to induce callus. The leaves obtained from seed tissue culture on Murashige and Skoog (MS) for 4 weeks were induced callus formation in MS with 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 0.5, 1.0, and 2.0 mg/L for 4 weeks. The results showed that all experiments could induce callus, and at the concentration 1.0 mg/L 2,4-D the largest callus, green color and compact callus was produced. Therefore, the data of this research can be used as information for the production of secondary metabolites.