Effect of 2,4-D and Kinetin on Callus Induction from in vitro Leaf Explant of Sundews (Drosera spatulata Labill.)
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Abstract
Callus culture is one of techniques that could be utilized to produce secondary metabolites from sundews (Drosera spatulata Labill.). This research aims to study the effect of 2,4-D and kinetin on callus induction from in vitro leaf explant of sundews. The leaf explants were cultured on ½ MS medium supplemented with 0, 0.5, 1 and 2 mg/L 2,4-D alone, or in combination with 0 and 0.2 mg/L kinetin under dark conditions for 6 weeks. The experimental design followed a completely randomized design (CRD) with seven treatments and three replications. The results showed that shoots were induced on ½ MS medium without plant growth regulators, while callus was induced on ½ MS medium supplemented with 2,4-D alone, or in combination with kinetin. The callus formation percentages ranged from 83.33 ± 7.22 to 87.5 ± 0 % on ½ MS medium supplemented with 0.5–2 mg/L 2,4-D in combination with 0.2 mg/L kinetin, which significantly differed from the other treatments. Furthermore, the highest fresh (475.95 ± 40.35 mg) and dry weight (58.10 ± 1.30 mg) were observed on ½ MS medium supplemented with 0.5 mg/L 2,4-D in combination with 0.2 mg/L kinetin. The compact and yellowish red color callus was induced from this study.
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