Effect of 2,4-D and Kinetin on Callus Induction from in vitro Leaf Explant of Sundews (Drosera spatulata Labill.)

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Kanokwan Sangmukdee
Panumart Rithichai
Yaowapha Jirakiattikul


Callus culture is one of techniques that could be utilized to produce secondary metabolites from sundews (Drosera spatulata Labill.). This research aims to study the effect of 2,4-D and kinetin on callus induction from in vitro leaf explant of sundews. The leaf explants were cultured on ½ MS medium supplemented with 0, 0.5, 1 and 2 mg/L 2,4-D alone, or in combination with 0 and 0.2 mg/L kinetin under dark conditions for 6 weeks. The experimental design followed a completely randomized design (CRD) with seven treatments and three replications. The results showed that shoots were induced on ½ MS medium without plant growth regulators, while callus was induced on ½ MS medium supplemented with 2,4-D alone, or in combination with kinetin. The callus formation percentages ranged from 83.33 ± 7.22 to 87.5 ± 0 % on ½ MS medium supplemented with 0.5–2 mg/L 2,4-D in combination with 0.2 mg/L kinetin, which significantly differed from the other treatments. Furthermore, the highest fresh (475.95 ± 40.35 mg) and dry weight (58.10 ± 1.30 mg) were observed on ½ MS medium supplemented with 0.5 mg/L 2,4-D in combination with 0.2 mg/L kinetin. The compact and yellowish red color callus was induced from this study.


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Biological Sciences


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