Development of Rapid Detection of Staphylococcus aureus in Water by Real-time PCR Technique
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Abstract
The important microorganisms that contaminate water include Staphylococcus aureus, Escherichia coli, and Salmonella sp., with a particular emphasis on the extensively studied S. aureus, commonly found on the skin, in the respiratory tract, and in human wounds. This research focuses on the development of real-time PCR technology for the detection of S. aureus in water. Real-time PCR, known for its specificity to individual pathogen DNA and high sensitivity, enables quantitative detection of targeted pathogens with minimal sample quantities. The method allows testing at contamination levels as low as 4200 CFU/mL without additional processing. Remarkably, the detection process takes only about 2 hours. This study underscores the heightened sensitivity and specificity of the developed real-time PCR technology, making it a viable and efficient method for detecting S. aureus in water samples.
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