Antioxidant activity and Preliminary Phytochemical Screening of Sunpatong Sticky Rice Extract from Ban Sanpong, Mae Rim District, Chiang Mai Province.
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Abstract
This study aimed to test the antioxidant activity and conduct a preliminary phytochemical screeningof Sunpatong sticky rice extract. The rice samples used for extraction were cultivated and harvested from Ban Sanpong, Mae Rim District, Chiang Mai Province.The extraction method employed was maceration, where the rice samples were soak in deionized water at room temperature. Four extraction methods were utilized: (1) soaking the rice for 24 hours followed by evaporation (RE24), (2) soaking the rice for 48 hours followed by evaporation (RE48), (3) soaking the rice for 24 hours followed by freezing at -20°C (RW24), and (4) soaking the rice for 48 hours followed by freezing at -20°C (RW48). The resulting extracts were characterized as crude extracts and solutions. The antioxidant properties were evaluated through the DPPH radical scavenging and ABTS•+ methods, and preliminary phytochemical screening was performed, whichincluded examining for tannins and terpenoids.Thetest results indicated that the RE24 extract exhibited thehighest level of antioxidant activity. In preliminary phytochemical screening analysis,noneof the four extracts contained tannins or terpenoids. However, the RE24 extract had the highest total flavonoid content (1.62 ± 0.09 mg CE/g extract), while the RE48 extract had the highest total phenolic content (9.99 ± 0.12 mg GAE/g extract)
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