Effects of Chlorine Dioxide and Sodium Hypochlorite in Culture Medium on Micropropagation of Pineapple (Ananas comosus) cv. Phulae
Article Sidebar
Main Article Content
Abstract
Plant tissue culture is widely used for pineapple propagation due to its ability to produce many plantlets but the cost of media sterilization through autoclaving is expensive. Medium supplemented with disinfectants can achieve sterility and cost reduction. However, these disinfectants could affect shoot multiplication and root induction of pineapple grown under aseptic conditions. Therefore, the objective of this study was to investigate the effect of various concentrations of sodium hypochlorite (NaOCl) and chlorine dioxide (ClO2) on shoot and root induction of pineapple cv. Phulae. In vitro shoots were cultured for 6 weeks on MS medium supplemented with 1 mg/L benzyladenine (BA) for shoot induction and on MS medium supplemented with 2 mg/L 1-naphthaleneacetic acid (NAA) for root induction. NaOCl at concentrations of 0.5, 1 and 2 mL/L and ClO2 at concentrations of 50, 75 and 100 mg/L were added to the medium for sterilization, compared to autoclaving (control). The experiments were arranged in a completely randomized design with 7 treatments and 4 replications. The results indicated that a 100% sterilized medium was obtained by adding NaOCl and ClO2. Furthermore, the application of 1-2 mL/L NaOCl and 50-100 mg/L ClO2 did not affect shoot and root induction. The number of shoots ranged from 3.81±0.53 to 5.16±0.33, the number of roots varied from 19.88±0.96 to 21.41±1.33, and root length ranged from 1.29±0.11 to 1.75±0.37 cm. After transplanting the plantlets, a 100% survival rate was achieved.
Article Details
References
Office of agricultural economics. Pineapple. Agricultural product-pineapple. source: http://mis-app.oae.go.th/product/สับปะรด. 17 October 2022
Office of agricultural economics. 2022. Guidelines for creating zones to promote the cultivation of important economic crops. Ministry of Agriculture and Cooperatives.
Thu, S. L., Tam, H. L. M., Tongdeesoontorn, W. and Suthiluk, P. 2017. Quality changes and volatile compounds in fresh cut ‘Phulae’ pineapple during cold storage. Curr. Appl. Sci. Technol. 17: 161-171.
Panyamongkol, S. 2017. The relationship between physical and chemical properties in Poolae pineapple with maturity stage among 0-7. Kasalongkham Research Journal 11: 49-59. (in Thai)
Ampawan, R., Pookmanee, T., Sumransakul, P., Duangbal, D., Taeja, S and Wongchai, K. 2016. Industrial production of pineapple seedlings. The office of agricultural research and extension. Maejo University, Chang Mai. (in Thai)
Usman, I. S., Abdulmalik, M. M., Sani, L. A. and Muhammad, A. N. 2013. Development of an efficient protocol for micropropagation of pineapple (Ananas comosus L. var. smooth cayenne). Afr. J. Agric. Res. 8: 2053-2056.
Nelson, B. J., Asare, P. A. and Junior, R. A. 2015. In vitro growth and multiplication of pineapple under different duration of sterilization and different concentrations of benzylaminopurine and sucrose. Biol. 14: 35-40.
Atawia, A. R., EL-Latif, F. M. A., EL-Gioushy, S. F., Sherif, S. S. and Kotb, O. M. 2016. Studies on micropropagation of pineapple (Ananas comosus L.). Middle East J. Agric. Res. 5 (2): 224-232.
Srichuay, W., Promchan, T. and Te-chato, S. 2018. Effect of chlorine dioxide (ClO2) on culture medium sterilization on micropropagation of persian violet (Exacum affine Balf.f. ex Regel). Int. J. Agric. Technol. 14: 259-270.
Wamaedeesa, R., Ali, B., Chedao, N., Kanjanawattanawong, S. 2021. Chemical sterilization in MS culture medium for in vitro culture of Philodendron sp. “Ruaysap”. Princess of Naradhiwas University Journal 13: 377-387. (in Thai)
Kasam, W., Wangwibulkit, M. and Laohavisuti, N. 2020. Effect of chlorine dioxide (ClO2) on sterilization in micropropagation of Anubias sp. ‘White’. J. Fish. Tech. Res. 14: 65-72. (in Thai)
Russell, A. D. 2003. Similarities and differences in the responses of microorganisms to biocides. J. Antimicrob. Chemother. 52: 750-763.
Deein, W., Thepsithar, C., Thongpukdee, A. and Tippornwong, S. 2013. Growth of chrysanthemum explants on MS medium sterilized by disinfectants and essential oils. Int. J. Biosci. Biochem. Bioinforma. 3: 609-613.
Peiris, S. E., De Silva, E. D. U. D., Edussuriya, M., Attanayake, A. M. U. R. K. and Peiris, B. C. N. 2012. CSUP technique: a low-cost sterilization method using sodium hypochlorite to replace the use of expensive equipment in micropropagation. J. Natn. Sci. Foundation Sri Lanka 40:49-54.
Teixeira, S. L. 2006. The development of a new protocol that uses sodium hypochlorite to replace the autoclaving procedure for establishing axenic in vitro banana (Musa sp.) culture. Agricell Report 47: 17-18.
Matsumoto, K., Coelho, M. C., Momte, D. C. and Teixera, J. B. 2009. Sterilization of non-autoclavable vessels and culture media by sodium hypochlorite for in vitro culture. Acta Hort. 839: 329-336.
Huang, J., Wang, L., Ren, N., Ma, F. and Ma, J. 1997. Disinfection effect of chlorine dioxide on bacteria in water. Water Res. 31: 607-613.
Tachapinyawat, S. 1995. Plant physiology. Rua Khiao Publisher, Bangkok. 213 p. (in Thai)