Genetic Relationships Assessment and Identification of Nepenthes and Their Hybrids Using SCoT Technique
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Abstract
The pitcher plant (Nepenthes) is a carnivorous species with high ornamental value due to the aesthetic appeal of its pitchers. This makes it a popular choice for decorative purposes and plant collections. However, identifying Nepenthes based solely on morphological characteristics can be challenging because many hybrids closely resemble one another. This study employed the Start Codon Targeted (SCoT) marker technique to differentiate 15 Nepenthes varieties, including eight species and seven hybrids. Of the 80 SCoT primers tested, 19 produced clear and highly polymorphic amplification profiles. Polymorphic primers were selected based on band clarity and the degree of polymorphism observed. Genetic relationships were analyzed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), revealing similarity coefficients ranging from 0.47 to 0.86. The samples were grouped into six distinct clusters.
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References
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