Influence of NaCl on Growth of Nymphaea ‘Wanwisa’ in in vitro Culture
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Abstract
The effect of sodium chloride on the growth of Nymphaea ‘Wanwisa’ under sterile conditions for the selection of salt-tolerant lines was studied. Shoot fragments of Nymphaea ‘Wanwisa’ were cultured in MS liquid medium supplemented with 0.1 mg/L TDZ, 4 mg/L NAA, and sodium chloride at concentrations of 0, 25, 50, 75, and 100 mM. The experiment was arranged in a completely randomized design with 5 treatments and 8 replications each, and the cultures were maintained under sterile conditions for 4 months. The results showed that shoots of Nymphaea ‘Wanwisa’ grew well in the media supplemented with 25 and 50 mM sodium chloride, with no significant differences compared to the control medium without sodium chloride. Shoot growth was significantly reduced (P<0.05) when cultured in media containing 75 and 100 mM sodium chloride. These findings indicate that the shoots can grow normally in media supplemented with up to 50 mM sodium chloride, suggesting a tendency toward salt tolerance. Therefore, the results from this experiment provide a guideline for future development and production of salt-tolerant Nymphaea ‘Wanwisa’ under sterile conditions.
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References
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