Effects of Extraction Solvents on the Recovery and Isolation of Pinostrobin, Pinocembrin, and Panduratin A from Boesenbergia rotunda Rhizomes
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Abstract
Fingerroot (Boesenbergia rotunda L. Mansf.) is an important source of flavonoids and chalcones with potential applications in pharmaceuticals, nutraceuticals, and the quality control of herbal products. This study evaluated the effects of solvent polarity on extract yield and the contents of pinostrobin, pinocembrin, and panduratin A, as well as the feasibility of scaling up the extraction process and systematically isolating bioactive compounds. The results showed that ethanol produced the highest total extract yield, whereas dichloromethane exhibited the greatest selectivity for pinostrobin and pinocembrin, while ethanol showed the greatest selectivity for panduratin A. Ethanol was therefore selected for scale-up extraction. All three target compounds were subsequently isolated using column chromatography combined with crystallization, and their structures were confirmed by NMR spectroscopy. These findings demonstrate that solvent selection for fingerroot extraction should consider both total extract yield and selectivity for the target compounds. The knowledge gained bridges laboratory-scale solvent selection and scaleup extraction and purification, providing a foundation for developing an efficient target-oriented extraction process suitable for further applications.
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References
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