QTL Mapping of Yield Related Components in Double Haploid (DH) Pepper Population Derived from F1 of ‘PEPAC7’× ‘PEPAC92’ Cultivars

Abstract

Association of genotypes by single nucleotide polymorphism (SNP) markers and phenotype of yield-related traits, i.e. fruit weight (Fw), fruit length (Fl), fruit width (Fwi), fruit peduncle length (Fpl), and fruit wall thickness (Fwt) were evaluated in 101 lines double-haploid population together with parental lines (PEPAC7 and PEPAC92) and F1 hybrid. Genotypic data was analyzed using the genotype-by-sequencing technique DArTSeq (DArT P/L). Nine thousand and twenty-five SNPs were obtained and then filtered by PIC value (≥ 0.8) and segregation ratio of 1 : 1 resulting in 2,572 SNP markers to be used in linkage analysis. The genetic map consisted of 12 linkage group using JoinMap version 4.1 was constructed. The single marker analysis and MQM mapping for traits were analyzed in MapQTL version 6 software. QTLs associated with the fruit weight trait were identified as qFw1.1, qFw1.2 and qFw10.1, on linkage groups 1 and 10, fruit length as qFl3.1, qFl3.2, and qFl3.3 on linkage group 3, fruit width as qFwi3.1, qFwi3.2, qFwi3.3, and qFwi2.1, on linkage groups 2 and 3. There are QTLs for fruit pedicel length identified as qFpl6.1, qFpl5.1, qFpl9.1 and qFpl11.1 on linkage groups 5, 6, 9 and 11 and for fruit wall thickness as qFwt6.1 and qFwt7.1 on linkage groups 6 and 7.