Comparison of Cassava Cleaned Seeds Producing Methods and Sampling Methods for Assessment of Cassava Mosaic Disease
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Abstract
This research aimed to compare the methods for cleaned seed production in nearly infected area and sampling methods for assessment of cassava mosaic disease. The randomized complete block design (RCBD) was applied to comparison of cleaned seed production. There were 4 methods with 4 replications including method 1: non-survey and non-rouging infected plants during plantation, method 2: survey and rouging infected plants when cassava at 2, 4, 5, 6, 7, 8 and 9 months, method 3: survey and rouging infected cassava plants when cassava at 2, 4, 7 and 9 months, and method 4: survey and rouging infected cassava plants when cassava at 2, 4 and 9 months. The result showed that disease incidence of methods 1, 2, 3, and 4 were 11.98 ± 1.35, 0.49 ± 0.06, 1.15 ± 0.26, and 1.68 ± 0.27% respectively, which were statistically significantly different (P < 0.05). The disease incidence of methods 2, 3 and 4
were less than 10% which were acceptable for cleaned seed production. Disease severity was moderate level in all methods. A comparison of sampling methods for disease assessment consisted of 2 methods including X-shape sampling and Q sampling. Disease incidence of X-shape sampling for disease assessment in methods 1, 2, 3 and 4 were 12.50 ± 2.15, 0.83 ± 0.96, 2.50 ± 0.96 and 5.00 ± 1.36%, respectively. Whereas disease incidence of Q sampling for disease assessment in methods 1, 2, 3 and 4 were 11.44 ± 1.13, 1.50 ± 0.46, 2.63 ± 0.52 and 3.00 ± 0.54%, respectively. Disease incidence of both sampling methods were not significantly different (P > 0.05). Disease detection using PCR technique presented that X-shape sampling and Q sampling in method 1 were 15.00 and 25.00%, respectively while methods 2, 3 and 4 were detected less than 10%. This research indicated that the survey and rouging infected plants when cassava at 2, 4 and 9 months and disease assessment with X-shape sampling and Q sampling might be appropriate procedures for producing cassava cleaned seed.
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