Efficiency of Trichoderma Fungus and Growing Media on Growth of Dendrobium sonia “Red Jo” Derived from Tissue Culture

Abstract

The efficiency of Trichoderma fungus and growing media on growth of Dendrobium sonia “Red Jo” derived from tissue culture, growing under greenhouse nursery with 60% of shading. The experiment was designed by 4 × 6 Factorial in RCBD with 10 replications, including with 4 concentrations of Trichoderma fungus powder of T. harzianum. (0, 1.25, 2.50 and 3.75 g/L) and 6 growing media formulas (coconut husk chips, perlite, pumice stone, 1 : 1 ratio of coconut husk chips and perlite, 1 : 1 ratio of coconut husk chips and pumice stone, and 1 : 1 : 1 ratio of coconut husk chips, perlite and pumice stone). The growth and development data of Dendrobium orchid micropropagations were collected at 1 month after transplanted. The result showed that, the interaction effect of Trichoderma fungus and growing media promoted plant height character of dendrobium orchid. The effect of coconut husk chips and perlite with 3.75 g/L of Trichoderma fungus powder have the highest plant height were 3.88 and 3.38 cm., respectively. The efficiency of Trichoderma fungus can increased the root number, plant height and leaf width of Dendrobium orchid, while growing media was affected on root length, plant height and leaf number. Correlation coefficients showed that the Trichoderma fungus had a moderately positive correlation with the plant height of Dendrobium orchid. According to this study, might be used to utilization improvement of Trichoderma fungus on Dendrobium or other commercial orchids production.