Effects of Media from Hydroponics Solution and Culture Temperature on Tissue Culture of Bacopa caroliniana (Walt.) Rob.

Abstract

This research had objective to study the effects of media from hydroponics solution and culture temperature on nodal culture of Bacopa caroliniana (Walt.) Rob. The explants were sterilized with 10% Haiter (6% sodium hypochlorite, NaoCl) for 15 min. Sterilized explants were cultured on media from hydroponic solutions (Hydrowork for salad vegetables) with two stocks A and B at the concentration 0, 1, 2, 3, 4 and 5 ml/L associated with 30 g/L sugar, 7 g/L agar and pH was adjusted to 5.7 which were sterilized with 0.5 mL/L Haiter supplementation. They were incubated in 16 h photoperiod at 25 ± 2ºC compared with room temperature for 4 weeks. It was found that all treatments induced shoot production (1.69–1.92 shoots per explant) and then all plantlets were transplanted into the greenhouse for 2 weeks. It was showed 100% survival rate of plantlet.