Development of Maize Inbred Lines by Doubled Haploid Method from S0 and S1 Populations

Abstract

Development of maize inbred lines by doubled haploid method using haploid inducer is lack of supporting data on selecting based population. The objective of this study was to compare efficiency of maize inbred line development by doubled haploid method from S₀ and S₁ populations. S₀ and S₁ populations of four populations (Q1, Q2, K3 and K4) were crossed with haploid inducer, PHI–3 inbred line. Haploid seeds, which expressed anthocyanin color marker in aleurone layer, were selected and germinated for four days. Then, the seedlings were selected for haploid (normal white root) again and treated with colchicine for 12 hours to double chromosome. After that, the seedlings were rinsed and transplanted to field plot. At flowering period, dihaploid plants with pollen shedding were selected and selfed to produce DHS₀–D1 and DHS₁–D1, respectively. The results showed that using S₀ and S₁ populations as based population gave haploid induction rate (HIR) of 5.4 and 6.4%, produced inbred lines for 25 and 128 lines, and used two and three seasons for inbred line development, respectively. Therefore, inbred line development by DHS₀ and DHS₁ methods can shorten the seasons used in inbred line development for eight and seven seasons as compared with conventional method. The results suggested that using S₁ population for crossing with haploid inducer can produce much more inbred lines as required.