The Efficiency of LEDs on In Vitro Growth, Antioxidant Activity and Phenolic Compound Content of Arundina graminifolia (D.Don) Hochr.
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Abstract
Bamboo orchid (Arundina graminifolia (D. Don) Hochr.) is a terrestrial orchid which has medicinal properties. This plant species growing in nature is widely used as the herbal plant in other countries. Thus, the objective of this study aims to compare the efficiency of different light qualities in vitro culture on growth, antioxidant activity, and total phenolic compounds content of bamboo orchid seedlings. Seedlings were cultured on semi-solid modified Vacin and Went medium (VW) supplemented with 10 g L-1 sucrose, 150 mL L-1 coconut water, 2 g L-1 activated charcoal, 100 g L-1 mashed banana, and 100 g L-1 boiled potato water under different light quality. The experimental design was a completely randomized design (CRD) with 5 treatments that included white, red, blue, and a mixture of red and blue LEDs (1:1) compared with fluorescent light (Control) under 40 μmol m–2s–1 in light intensity for 10 weeks. The results exhibited that light quality statistically affected growth,
antioxidant activity, and total phenolic content, except plant height, leaf number, leaf length, and root length. White LEDs induced the highest fresh weight and total chlorophyll content (1.30 g and 38.38 mg cm–2, respectively) of bamboo orchid seedlings. Furthermore, white LEDs also promoted and maximized antioxidant activity and total phenolic compound content (EC50 72.17 gFW/L and 1.39
mgGAE/gFW, respectively) while a mixture of red and blue LEDs (1:1) showed the greatest stem diameter and leaf width (0.36 and 0.45 cm, respectively). Therefore, white LEDs provide a guideline for the increase of secondary metabolite compounds of pharmaceuticals from bamboo orchid seedlings.
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