Effects of Rotifers Enriched with Three Different Types of Microalgae on Growth and Survival Rate of Diadema Larvae Pseudochromis diadema
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Abstract
In this study, we investigated the growth and survival rate of newly hatched diadema larvae (Pseudochromis diadema) fed with rotifers (Brachionus rotundiformis) supplemented with three types of microalgae (Tetraselmis gracilis, Isochrysis galbana, and Nanochloropsis oculata). The control group was fed with rotifers without any nutritional supplement. Each group was replicated three times for the experiment. The larvae were fed twice daily at 9:00 am and 3:00 pm with a density of 3 rotifers per milliliter for 15 days. At the end of the experiment, it was found that the experimental group 2 had the highest total length gain of 4.28 ± 0.10 mm (P<0.05) and the standard length was also the highest in group 2 (P<0.05). All larvae in the control group died after 3 days, while the survival rate was the highest in group 1 (P<0.05). The study compared the nutritional value and total fatty acids of three types of microalgae. It found that I. galbana had the highest protein content (P<0.05), N. oculata had the highest lipid content (P<0.05), and N. oculata had the highest total fatty acids content. When these microalgae were used as a supplement for rotifers, rotifers enriched with I. galbana had the highest lipid content, rotifers enriched with T. gracilis had the highest protein content (P<0.05), and N. oculata resulted in the highest total fatty acids content.
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