Effect of Fermented StarFruit (Averrhoa carambola L.) Bioextracton Seed Germination of Holland Papaya (Carica papaya L.)
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Abstract
This study aimed to investigate the effects of different dilution ratios of fermented star fruit (Averrhoa carambola L.) bioextract on seed germination and seedling growth of Holland papaya (Carica papaya L.). The experiment was arranged in a Completely Randomized Design (CRD) with four replications, consisting of five treatments: distilled water (control) and fermented bioextract diluted with distilled water at ratios of 1:250, 1:500, 1:750, and 1:1,000 (v/v). Holland papaya seeds were soaked in each treatment at room temperature for 16 hours, rinsed thoroughly, air-dried for 3 hours, and subsequently tested for germination and seedling growth in peat moss. After 28 days of sowing, results indicated that the 1:1,000 (v/v) ratio significantly produced the highest germination percentage (81.25%) and the shortest mean germination time (12.25 days) compared with other treatments (P ≤ 0.01). For seedling growth, the same dilution ratio also resulted in the greatest stem height (13.46 cm) and root length (5.94 cm), with statistically significant differences from other treatments (P ≤ 0.05). In conclusion, a 1:1,000 (v/v) dilution of fermented star fruit bioextract to distilled water was the most effective treatment, enhancing seed germination and promoting the early growth of Holland papaya seedlings.
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References
Nimkingrat, T., et al. (2021). Papaya Breeding Programm for Fresh Fruit (Research reports). Bangkok: Departmentof Agriculture. (in Thai)
Department of Internal Trade. (2024). Consumer Goods and Agricultural Product Prices. Ministry of Commerce. (in Thai)
Rungrueng, P., et al. (2019). Propagation of Papaya (Carica papaya L.) cv. Holland in Tissue Culture. Khon Kaen Agriculture Journal, 47(3), 459-466. (in Thai)
Sansuk, M. (2014). Papaya Is Making Rich Money. Nana Publishing. (in Thai)
Lange, W. (1991). Seed Germination: Factors Influencing the Process. Journal of Agricultural Science, 20(4), 112-118.
Bhattacharya, J., & Khuspe, S. S. (2001). In Vitro and In Vivo Germination of Papaya (Carica papaya L.) Seeds. Scientia Horticulturae, 91(1-2), 39-49.
Lange, A. H. (1961). Effect of Sarcotesta on the Germination of Papaya Seed. Botanical Gazette, 122(4), 305-311.
Yahiro, M. (1979). Effect of Seed Pre-Treatment on the Promotion of Germination in
Papaya (Carica papaya L). Memoris of the Faculty of Agriculture, Kagoshima University, 15, 49-54.
Horticultural Research Institute. (n.d.). Holland Sisaket papaya variety. Available from https://www.doa.go.th/hort/?page_id=54065. Accessed date: 25 August 2025. (in Thai)
Sukprakarn, S., & Prapanopasin, P. (2002). Hydropriming Treatments on Chilli Seeds Germination. Agricultural Science Journal, 33(4-5), 141-148. (in Thai)
Kunchon Na Ayudhya, V. (2001). Local Wisdom Technology. Department of Agriculture, Ministry of Agriculture and Cooperatives. (in Thai)
Department of Agriculture. (2004). Scientific Information on Bio-Fermented Liquid (Part 1) (Technical Document No. 3/2004). Department of Agriculture. (in Thai)
Chumpookam, J., et al. (2014). Effect of Bioextract From Coffee Pulp on Seed Germination and Seedling Growth of "Khaek Dam" Papaya (Carica papaya Linn.). In Proceedings of the 35th Kasetsart University Annual Conference (pp. 1-8). 3-5 February, 1997, Bangkok, Thailand. (in Thai)
Kitsanachandee, R. (2017). Effects of Liquid Bio-Extracts From Plants to Release of Dormancy in Lettuce Seed. Khon Kaen Agriculture Journal, 45(Suppl. 1), 1283-1288. (in Thai)
Kumplee, S. & Dumrongkittikule, S. (2019). Effect of Fermented Fruit Juice on Chilli Seed Germination. In Proceedings of the 9th Kasetsart University, Kamphaeng Saen Campus Conference. (p. 699-705). 6 – 7 December, 2019, ,Nakhon Pathom, Thailand. (in Thai)
Phonmat, S., et al. (2023). Effect of Water Hyacinth Fermented Bio-Extracts on Germination of Chili (Capsicum annuum L.). Journal of Science and Technology, Nakhon Sawan Rajabhat University, 15(1), 1-14. (in Thai)
Suwannakhiri, S. (2004). Bio-Liquid Fertilizer or Bio-Extract and Its Application With Effective Microorganisms (EM) in Agriculture and Environment (Research report). Chiang Mai: Chiang Mai University. (in Thai)
Lim, T. K. (2012). Averrhoa carambola (Carambola). Edible Medicinal and Non-Medicinal Plants. 2(5). 78–88.
Apidech, P. (2014). Antioxidant Activity of Fruits in the Family Oxalidaceae. Thai Journal of Science, 41(2), 45-52. (in Thai)
Kuntang, P., et al. (2022). Metabolite Profile, Antioxidant Activity and Anti-Candida Activity of Fermented Star Fruit Bioextract (Averrhoa carambola L.). Trends in Sciences, 19(24), 4183.
Bewley, J. D., & Black, M. (1994). Seeds: Physiology of Development and Germination. Plenum Press.
Taiz, L., & Zeiger, E. (2015). Plant Physiology (6th ed.). Sinauer Associates.
Gill, P. K., et al. (2003). Changes in Germination, Growth and Soluble Sugar Contents of Sorghum bicolor Seeds Under Various Abiotic Stresses. Plant Growth Regulation, 40(2), 157-162.
Günal-Köroğlu, D., et al. (2025). Effect of Germination on the Phenolic Compounds: Content, Bioavailability, Food Applications, and Health Benefits. Journal of Food Measurement and Characterization, 19, 8144-8164.
Jovanović, I., et al. (2024). Role of Total Polyphenol Content in Seed Germination Characteristics of Spring Barley Varieties Amidst Climate Change. Scientific Reports, 14, 23818.
American Public Health Association. (2017). Standard Methods for the Examination of Water and Wastewater (23rd ed.). APHA.
International Seed Testing Association. (2025). International Rules for Seed Testing. ISTA.
Ellis, R. H., & Roberts, E. H. (1980). Improved Equation for the Prediction of Seed Longevity. Annals of Botany, 45(1), 13-30.
Abdul-Baki, A. A., & Anderson, J. D. (1973). Vigor Determination in Soybean Seed by Multiple Criteria. Crop Science, 13(6), 630-633.
Tancho, A. (2006). Applied Natural Agriculture. National Science and Technology Development Agency. (in Thai)
Copeland, L. O., & McDonald, M. B. (1995). Principles of Seed Science and Technology (3rd ed.). Chapman & Hall.
Tokhun, N., & Worawut, K. (2021). Effects of Different Soaking Techniques on Germination and Seed Vigor of Kaowong Kalasin and Pathum Thani 1 Rice Varieties. Khon Kaen Agriculture Journal, 49(Suppl. 1), 942-948. (in Thai)
Chartzoulakis, K., & Klapaki, G. (2000). Effects of Salinity on Seed Germination, Growth and Yield of Greenhouse Cucumber. Journal of Horticultural Science & Biotechnology, 75(4), 499-504.
Kabar, K., & Fazal, A. (1981). Effect of pH on Seed Germination of Various Crop Species. Seed Science and Technology, 9(2), 535-540.
Kaya, C., et al. (2001). The Effects of Low Levels of Salinity on Seed Germination and Early Seedling Growth of Cucurbit Species. Scientia Horticulturae, 89(1), 47-54.
Munns, R., & Tester, M. (2008). Mechanisms of Salinity Tolerance. Annual Review of Plant Biology, 59, 651-681.
Yin, J., et al. (2025). Compost Tea: Preparation, Utilization Mechanisms, and Agricultural Applications Potential—A Comprehensive Review. Environmental Technology & Innovation, 38, 104137.
