Quantification of polyphenol content, antioxidant properties and LC-MS/MS analysis in Malaysian indigenous rice cultivars (Oryza sativa L.)

Authors

  • Norliza Abd Latiff Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Abd Rahman Jabir Mohd Din Innovation Centre in Agritechnology for Advanced Bioprocessing, UTM Pagoh Research Center, Pagoh Education Hub, 84600 Pagoh, Muar, Malaysia
  • Siti Atiqah Zainul Alam Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Siti Zulaiha Hanapi Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia
  • Mohamad Roji Sarmidi Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

Keywords:

Antioxidant properties, Bioactive compound, LC-MS/MS, Oryza sativa, Polyphenol contents

Abstract

The study was carried out to evaluate the differences in the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activities via 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic) and 2,2-diphenyl-1-picrylhydrazyl assays and quantification of targeted polyphenol compounds in Malaysian upland and wetland rice (Oryza sativa L. indica) varieties. Of note, the Bario cultivar of upland rice-seed grain extracts had the highest TPC (47.84 mg gallic acid equivalents/g), TFC (7.14 mg rutin equivalents/g) and antioxidant activities 25% higher than for the other cultivars. Ferulic acid, salicylic acid and p-coumaric acid, (the most abundant polyphenol compounds and known as a group of flavonoids responsible for high antioxidant properties) were detected in all extracts tested; upland rice of the Bario cultivar had a higher portion of ferulic acid (10.31 mg/100g), salicylic acid (3.25 mg/100g) and p-coumaric acid (1.10 mg/100g). This finding of antioxidant compounds was further confirmed using liquid chromatography combined with tandem mass spectrometry analysis in which ferulic acid produced the most substantial peak at [M-H]- m/z 193 to form the daughter ion of 134 at a retention time of 5.79 min, while p-coumaric acid was detected at m/z 163 to form the daughter ion of m/z 119 at a retention time of 4.95 min. The results indicated that the increased antioxidant quantity in upland rice could support the available upland rice grain database for the development of enriched health-promoting foods.

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Published

2019-08-30

How to Cite

Latiff, Norliza Abd, Abd Rahman Jabir Mohd Din, Siti Atiqah Zainul Alam, Siti Zulaiha Hanapi, and Mohamad Roji Sarmidi. 2019. “Quantification of Polyphenol Content, Antioxidant Properties and LC-MS/MS Analysis in Malaysian Indigenous Rice Cultivars (Oryza Sativa L.)”. Agriculture and Natural Resources 53 (4). Bangkok, Thailand:402-9. https://li01.tci-thaijo.org/index.php/anres/article/view/228753.

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Section

Research Article