Utilization of agricultural waste biomass by cellulolytic isolate Enterobacter sp. SUK-Bio

Authors

  • Pankajkumar R. Waghmare State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, PR China
  • Swapnil M. Patil Department of Biotechnology, Shivaji University, Kolhapur, 416004, India
  • Sanjivani L. Jadhav Department of Environmental Biotechnology, Shivaji University, Kolhapur, 416004, India
  • Byong-Hun Jeon Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea
  • Sanjay P. Govindwar Department of Biochemistry, Shivaji University, Kolhapur, 416004, India

Keywords:

Cellulolytic enzyme, Congo red, Enterobacter sp. SUK-Bio, Sorghum husk

Abstract

A cellulolytic bacterium was isolated from plant litter soil and identified as Enterobacter sp. SUK-Bio. This isolate was investigated for its utilization of different cellulosic materials (carboxymethyl cellulose, sugarcane trash, grass powder, sorghum husk, wheat straw and water hyacinth). Utilization of sorghum husk was comparatively more than for the other cellulosic materials used, producing higher cellulolytic and hemicellulolytic enzymes (filter paperase (0.15 U/mL), β -glucosidase (37.10 U/mL), endoglucanase (12.24 U/mL), exoglucanase (2.52 U/mL), xylanase (26.26 U/mL) and glucoamylase (33.26 U/mL)) on day 8 of incubation. Furthermore, it produced the maximum reducing sugar production (554 mg/L) at a rate of
3.84 mg/h/L. Fourier-transform infrared spectroscopy analysis of sorghum husk revealed functional groups changes and a decrease in the total crystallinity ratio after microbial degradation. The effects of supplementation of different metals additives, thermal stability and pH on cellulolytic enzymes were also studied.

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Published

2018-10-31

How to Cite

Waghmare, Pankajkumar R., Swapnil M. Patil, Sanjivani L. Jadhav, Byong-Hun Jeon, and Sanjay P. Govindwar. 2018. “Utilization of Agricultural Waste Biomass by Cellulolytic Isolate Enterobacter Sp. SUK-Bio”. Agriculture and Natural Resources 52 (5). Bangkok, Thailand:399-406. https://li01.tci-thaijo.org/index.php/anres/article/view/236498.

Issue

Vol. 52 No. 5 (2018): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.