Enzymatic Production of Fructo-Oligosaccharides from Sucrose

Authors

  • Sarote Sirisansaneeyakul Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Sittiwat Lertsiri Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • Preecha Tonsagunrathanachai Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Pairojana Luangpituksa Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Keywords:

fructo-oligosaccharides, Aspergillus niger ATCC 20611, β-fructofuranosidase

Abstract

To increase an efficiency of fructo-oligosaccharide production from sucrose, a mixed-enzyme system of β-fructofuranosidase (Aspergillus niger ATCC 20611) and glucose oxidase (Sigma Chemical Co., Ltd.) was carried out in a 2-l stirred tank reactor with the following controlled conditions; temperature 40°C, pH 5.5, aeration rate 1 vvm and agitation rate 550 rpm, for 32 hours of incubation time. With an initial sucrose concentration of 400 g/l, the optimal condition was obtained by using β-fructofuranosidase and glucose oxidase 10 and 15 units per gram sucrose, respectively. As a result, the yields of kestose (YGF2/S) and nystose (YGF3/S) from sucrose and their volumetric productivities (QGF2/S and QGF3/S) were 0.44, 0.49 g/g, and 4.97, 5.44 g/l h, respectively.

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Published

2000-06-30

How to Cite

Sirisansaneeyakul, Sarote, Sittiwat Lertsiri, Preecha Tonsagunrathanachai, and Pairojana Luangpituksa. 2000. “Enzymatic Production of Fructo-Oligosaccharides from Sucrose”. Agriculture and Natural Resources 34 (2). Bangkok, Thailand:262-69. https://li01.tci-thaijo.org/index.php/anres/article/view/240361.

Issue

Vol. 34 No. 2 (2000): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.