In Vitro Preservation of Vanda coerulea Giff. Protocorm-Like Bodies

Authors

  • Surawit Wannakrairoj Laboratory for Plant Variety Development, Department of Horticulture, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand

Keywords:

vanda, preservation, reduced - growth, in vitro, orchid

Abstract

Protocorm-like bodies (plbs) of Vanda coerulea were cultured on an agar medium consisted of halfstrength Vacin and Went (1949) macronutrient, half-strength Murashige and Skoog (1962) micronutrient and 150 ml/l coconut water. After 7 days of culture, plbs were overlaid with 5 cm paraffin-oil or embedded under 1 and 2 cm of water agar medium. The cultures were then incubated either in dark or under 17-25 mol/ m2/s of continuous light from "Day light" fluorescent lamps at 26±2°C. After 168 days of preservation, the plbs embedded under 2 cm agar gave the best result both in term of their appearance and their ability to regrow. This is the first report on agar-embedding conservation in vitro.

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Published

1998-09-30

How to Cite

Wannakrairoj, Surawit. 1998. “In Vitro Preservation of Vanda Coerulea Giff. Protocorm-Like Bodies”. Agriculture and Natural Resources 32 (3). Bangkok, Thailand:329-32. https://li01.tci-thaijo.org/index.php/anres/article/view/240581.

Issue

Vol. 32 No. 3 (1998): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.