Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture

Authors

  • Nitsri Sangduen Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Ampai Sinpatananon Nakhon Ratchasima Sericulture Research Center

Keywords:

mulberry (Morus sp.), colchicine, tetraploid induction, tissue culture

Abstract

Tetraploid induction of three mulberry (Morus sp.) varieties Noi, Khunpai and Yai Burirum was obtained from colchicine treatment by soaking aseptic mulberry nodes from tissue culture in 0.02 - 0.10 % colchicine added with 2% dimethyl sulfoxide (DMSO) solution for 2-5 days. Tetraploid plants were characterized by some indices e.g. thick and dark green leaves, increasing stomatal size and quantity of stomatal chloroplast, decreasing frequency of stomatal per unit area. The chromosome number of root tip cell was 2n = 4x = 56. Tetraploid mulberry attained from this study could be used to cross with diploid plant for further triploid
production.

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Published

1998-12-31

How to Cite

Sangduen, Nitsri, and Ampai Sinpatananon. 1998. “Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture”. Agriculture and Natural Resources 32 (4). Bangkok, Thailand:424-30. https://li01.tci-thaijo.org/index.php/anres/article/view/240597.

Issue

Vol. 32 No. 4 (1998): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.