Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture

บทคัดย่อ

Tetraploid induction of three mulberry (Morus sp.) varieties Noi, Khunpai and Yai Burirum was obtained from colchicine treatment by soaking aseptic mulberry nodes from tissue culture in 0.02 - 0.10 % colchicine added with 2% dimethyl sulfoxide (DMSO) solution for 2-5 days. Tetraploid plants were characterized by some indices e.g. thick and dark green leaves, increasing stomatal size and quantity of stomatal chloroplast, decreasing frequency of stomatal per unit area. The chromosome number of root tip cell was 2n = 4x = 56. Tetraploid mulberry attained from this study could be used to cross with diploid plant for further triploid
production.