Agrobacterium Mediated Transformation of Sri Sumrong 60, a Thai Cotton Variety

Authors

  • H. Chair CIRAD - DORAS Certer/PGEU, Kasetsart University, Nakhon Pathom 73140, Thailand
  • R. Kuhapituktum PGEU, Kasetsart University, Nakhon Pathom 73140, Thailand
  • T. Attathom Department of Entomology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • C. Pannetier CIRAD-CA, Laboratoire de Biologie cellulaire, Centre INRA, 78026Versailles Cedex-France
  • S. Attathom PGEU, Kasetsart University, Nakhon Pathom 73140, Thailand

Keywords:

Bacillus thuringiensis, Agrobacterium tumefaciens, cotton, somatic embryogenesis, crylA(b)

Abstract

Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyls explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic CryIA (b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of cryIA (b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60.

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Published

1997-12-31

How to Cite

Chair, H., R. Kuhapituktum, T. Attathom, C. Pannetier, and S. Attathom. 1997. “Agrobacterium Mediated Transformation of Sri Sumrong 60, a Thai Cotton Variety”. Agriculture and Natural Resources 31 (5). Bangkok, Thailand:149-56. https://li01.tci-thaijo.org/index.php/anres/article/view/241067.

Issue

Vol. 31 No. 5 (1997): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.