Agrobacterium Mediated Transformation of Sri Sumrong 60, a Thai Cotton Variety

บทคัดย่อ

Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyls explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic CryIA (b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of cryIA (b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60.