Rice Improvement Through Immature Embryo Culture and Colchicine Treatment

Authors

  • Pradit Pongtongkam Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Surin Peyachoknagul Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Paderm Ratisoontorn Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Saowanee Suputtitada Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Kanchana Klakhaeng Pathumtani Rice Research Center, Amphor Klong Luang, Pathumthani 12120, Thailand.
  • Nitsri Sangduen Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Chairerk Maneepongse Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Songkran Jitrakorn Pathumtani Rice Research Center, Amphor Klong Luang, Pathumthani 12120, Thailand.

Keywords:

rice improvement, immature embryo rescue

Abstract

The F1 hybrids (IR 70/O. minuta and IR 72/O. minuta) were cultured on the modified MS media containing 15% coconut water, 1 g/l yeast extract, 3 mg/l BAP and 0.01, 0.03 and 0.05% colchicin during 3, 5 and 7 days. The F1 hybrids could have most rapid growth and many tillers on the 0.01% colchicine supplemented medium. These F1 hybrids from such medium which were treated for 3, 5 and 7 days were transferred to pots in the greenhouse. Some tillers produced panicles and seeds, while some tillers produced panicles but no seed formation. F2 seeds were planted, almost of tillers produced panicles and seeds. The size of F3 seeds were similar to cultivated varieties (IR 70 and IR 72).

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Published

1994-06-30

How to Cite

Pradit Pongtongkam, Surin Peyachoknagul, Paderm Ratisoontorn, Saowanee Suputtitada, Kanchana Klakhaeng, Nitsri Sangduen, Chairerk Maneepongse, and Songkran Jitrakorn. 1994. “Rice Improvement Through Immature Embryo Culture and Colchicine Treatment”. Agriculture and Natural Resources 28 (2). Bangkok, Thailand:193-99. https://li01.tci-thaijo.org/index.php/anres/article/view/241393.

Issue

Vol. 28 No. 2 (1994): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.