Rapid Method in Identification of Clostridium perfringens in Foods by Reverse CAMP Test

Authors

  • Siriporn Stonsaovapak Institute of Food Research and Product Development, Kasetsart University, Bangkok 10903, Thailand.
  • Supannee Chitpinityol Biological Science Division, Department of Science Service, Bangkok 10400, Thailand.
  • Wannee Somporn Biological Science Division, Department of Science Service, Bangkok 10400, Thailand.

Keywords:

Clostridium perfringens, identification, reverse CAMP test, rapid method

Abstract

Ninety-seven percent of Clostridium perfringens isolated from various kinds of food were reverse CAMP test positive. C. perfringens was detected by inoculation at right angles to within 1 to 2 mm. of a β-hemolytic group B Streptococcus agalactiae on sheep blood agar. A positive test was indicated by the formation of an arrowhead-shaped hemolytic pattern at the junction of the two cultures. The reverse CAMP test significantly reduces the time and cost for identifying C. perfringens frequently isolated from food.

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Published

1993-06-30

How to Cite

Siriporn Stonsaovapak, Supannee Chitpinityol, and Wannee Somporn. 1993. “Rapid Method in Identification of Clostridium Perfringens in Foods by Reverse CAMP Test”. Agriculture and Natural Resources 27 (2). Bangkok, Thailand:204-10. https://li01.tci-thaijo.org/index.php/anres/article/view/241721.

Issue

Section

Research Article