Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells

Authors

  • Sonthichai Chanprame Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Rewat Lersrutaiyotin Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Chongkolnee Weerasathakul Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

cryoprotectant, cryopreservation, sugarcane, cell suspension

Abstract

Cell suspension of sugarcane cultivar ROC3 treated with different concentration and combination of cryoprotectant were cryopreserved. Before cryopreservation, temperature of cell suspension was reduced from room temperature to 0°C/ min. and from 0°C to -40°C at the rate of 0.5°C/min.. Then, cell suspension were storaged in liquid nitrogen for 30 minutes. After storage, sugarcane cells were recultured again on solid media. Sugarcane cells treated with 15% dimethysulphoxide (DMSO) and 1.OM sucrose showed the best regrowth among the cryoprotectants studied.

Downloads

Published

1993-12-31

How to Cite

Sonthichai Chanprame, Rewat Lersrutaiyotin, and Chongkolnee Weerasathakul. 1993. “Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:1-3. https://li01.tci-thaijo.org/index.php/anres/article/view/241730.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.