Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells

ผู้แต่ง

  • Sonthichai Chanprame Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Rewat Lersrutaiyotin Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Chongkolnee Weerasathakul Dept, of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

คำสำคัญ:

cryoprotectant, cryopreservation, sugarcane, cell suspension

บทคัดย่อ

Cell suspension of sugarcane cultivar ROC3 treated with different concentration and combination of cryoprotectant were cryopreserved. Before cryopreservation, temperature of cell suspension was reduced from room temperature to 0°C/ min. and from 0°C to -40°C at the rate of 0.5°C/min.. Then, cell suspension were storaged in liquid nitrogen for 30 minutes. After storage, sugarcane cells were recultured again on solid media. Sugarcane cells treated with 15% dimethysulphoxide (DMSO) and 1.OM sucrose showed the best regrowth among the cryoprotectants studied.

ดาวน์โหลด

เผยแพร่แล้ว

1993-12-31

รูปแบบการอ้างอิง

Sonthichai Chanprame, Rewat Lersrutaiyotin, และ Chongkolnee Weerasathakul. 1993. “Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:1-3. https://li01.tci-thaijo.org/index.php/anres/article/view/241730.

ฉบับ

ปีที่ 27 ฉบับที่ 5 (1993): January-December

ประเภทบทความ

Research Article

สัญญาอนุญาต

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
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