Prospection for Using RAPD Analysis for Varietal Identification in Tomato and Cucumber

Authors

  • Panie Temiesak Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Pornpan Pooprompan Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Malinee Limsomboonchai Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.

Keywords:

RAPD, tomato, cucumber, variety, identification

Abstract

DNA extracted from leaves of seedlings was assessed using polymerase chain reaction (PCR) with single 10- base oligonucleotide random primers to analyse genetic differences among varieties of tomato and cucumber. Electrophoresis of amplified product revealed polymorphism among genetically related varieties. The RAPD (random amplified polymorphic DNA) analysis indicated a higher similarlity between Seeda Tip 93 to Seeda the two Tip 92 than Seeda Yak tomato variety, and higher similarlity between C4 cucumber improved varieties than the two local variety.

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Published

1993-12-31

How to Cite

Panie Temiesak, Pornpan Pooprompan, and Malinee Limsomboonchai. 1993. “Prospection for Using RAPD Analysis for Varietal Identification in Tomato and Cucumber”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:52-56. https://li01.tci-thaijo.org/index.php/anres/article/view/241740.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.