Prospection for Using RAPD Analysis for Varietal Identification in Tomato and Cucumber

ผู้แต่ง

  • Panie Temiesak Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Pornpan Pooprompan Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Malinee Limsomboonchai Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.

คำสำคัญ:

RAPD, tomato, cucumber, variety, identification

บทคัดย่อ

DNA extracted from leaves of seedlings was assessed using polymerase chain reaction (PCR) with single 10- base oligonucleotide random primers to analyse genetic differences among varieties of tomato and cucumber. Electrophoresis of amplified product revealed polymorphism among genetically related varieties. The RAPD (random amplified polymorphic DNA) analysis indicated a higher similarlity between Seeda Tip 93 to Seeda the two Tip 92 than Seeda Yak tomato variety, and higher similarlity between C4 cucumber improved varieties than the two local variety.

ดาวน์โหลด

เผยแพร่แล้ว

1993-12-31

รูปแบบการอ้างอิง

Panie Temiesak, Pornpan Pooprompan, และ Malinee Limsomboonchai. 1993. “Prospection for Using RAPD Analysis for Varietal Identification in Tomato and Cucumber”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:52-56. https://li01.tci-thaijo.org/index.php/anres/article/view/241740.

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ประเภทบทความ

Research Article