Improvement of Transformation Efficiency of Agrobacterium Mediated Gene Transfer in Tomato

Authors

  • Supat Attathom Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Petcharat Siriwong Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Wichai Kositratana Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Thira Sutabutra Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Abstract

The transformation/regeneration system was developed for Agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon disc transformation yielded 1-10 plantlets per tissue within 2-3 weeks. Transgenic tomato plants expressed kanamycin resistant gene and GUS (β-glucuronidase) activity.

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Published

1991-12-31

How to Cite

Supat Attathom, Petcharat Siriwong, Wichai Kositratana, and Thira Sutabutra. 1991. “Improvement of Transformation Efficiency of Agrobacterium Mediated Gene Transfer in Tomato”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:15-20. https://li01.tci-thaijo.org/index.php/anres/article/view/242008.

Issue

Vol. 25 No. 5 (1991): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.