Improvement of Transformation Efficiency of Agrobacterium Mediated Gene Transfer in Tomato

ผู้แต่ง

  • Supat Attathom Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Petcharat Siriwong Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Wichai Kositratana Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Thira Sutabutra Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

บทคัดย่อ

The transformation/regeneration system was developed for Agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon disc transformation yielded 1-10 plantlets per tissue within 2-3 weeks. Transgenic tomato plants expressed kanamycin resistant gene and GUS (β-glucuronidase) activity.

ดาวน์โหลด

เผยแพร่แล้ว

1991-12-31

รูปแบบการอ้างอิง

Supat Attathom, Petcharat Siriwong, Wichai Kositratana, และ Thira Sutabutra. 1991. “Improvement of Transformation Efficiency of Agrobacterium Mediated Gene Transfer in Tomato”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:15-20. https://li01.tci-thaijo.org/index.php/anres/article/view/242008.

ฉบับ

ปีที่ 25 ฉบับที่ 5 (1991): January-December

ประเภทบทความ

Research Article

สัญญาอนุญาต

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