Increasing the Glucoamylase Producing capacity at High Temperature of Yeast by Mutation

Authors

  • Savitree Limtong Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Touchaporn Rupasut Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Abstract

Isolation and selection of amylolytic yeast at 40oC resulted 3 effective strains, strain SP1, SP2 and SP3, that could produce higher concentration of glucoamylase that the others. The strain SP3 produced maximum glucoamylase as 120.15 units/ml which was the highest among these 3 strains. There 3 selected strains were subjected to mutagenesis using ethylmethanesulfonate with the result that among all mutants obtained, the mutant SP3MO8, a mutant of strain SP3, produced the highest concentration of glucoamylase at 180.72 units/ml. This concentration was 50.14% higher than that produced by the wild type strain SP3. However, the mutant SP2MO7 can increase the glucoamylase production at 61.17% higher that its wild type SP2.

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Published

1990-03-31

How to Cite

Savitree Limtong, and Touchaporn Rupasut. 1990. “Increasing the Glucoamylase Producing Capacity at High Temperature of Yeast by Mutation”. Agriculture and Natural Resources 24 (1). Bangkok, Thailand:76-86. https://li01.tci-thaijo.org/index.php/anres/article/view/242103.

Issue

Vol. 24 No. 1 (1990): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.