Increasing the Glucoamylase Producing capacity at High Temperature of Yeast by Mutation

บทคัดย่อ

Isolation and selection of amylolytic yeast at 40^oC resulted 3 effective strains, strain SP1, SP2 and SP3, that could produce higher concentration of glucoamylase that the others. The strain SP3 produced maximum glucoamylase as 120.15 units/ml which was the highest among these 3 strains. There 3 selected strains were subjected to mutagenesis using ethylmethanesulfonate with the result that among all mutants obtained, the mutant SP3MO8, a mutant of strain SP3, produced the highest concentration of glucoamylase at 180.72 units/ml. This concentration was 50.14% higher than that produced by the wild type strain SP3. However, the mutant SP2MO7 can increase the glucoamylase production at 61.17% higher that its wild type SP2.