Preparation of a local Strain of Mycoplasma gallisepticum Antigen for Rapid Serum Plate Agglutionation Test

Authors

  • Prapahd Neramitmansook Veterinary Research Division, National Animal Health and Production Institute, Department of Livestock Development, Bangkok 10400, Thailand.
  • Wantanee Neramitmansook Veterinary Research Division, National Animal Health and Production Institute, Department of Livestock Development, Bangkok 10400, Thailand.
  • Pairoj Minden Veterinary Research Division, National Animal Health and Production Institute, Department of Livestock Development, Bangkok 10400, Thailand.
  • Chadej Dumrongsiri Veterinary Research Division, National Animal Health and Production Institute, Department of Livestock Development, Bangkok 10400, Thailand.
  • Lanoppadol Devakul Veterinary Research Division, National Animal Health and Production Institute, Department of Livestock Development, Bangkok 10400, Thailand.

Abstract

Antigen of Mycoplasma gallisepticum (MG) for rapid serum agglutionation was prepared by using a local strain of MG which had been isolated from infected layers of a certain farm. The organism was grown in PPLO broth at least thirty-two passages after being selected at each passage for a pure colony. The antigen was demonstrated for rapid agglutination test by reacted chicken sera that has been experimentally infected with the same MG strain. The results indicated that the prepared antigens were 100% positive with tested sera, the same as with the commercial RSA antigen made from MG S 6 strain.

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Published

1989-09-30

How to Cite

Prapahd Neramitmansook, Wantanee Neramitmansook, Pairoj Minden, Chadej Dumrongsiri, and Lanoppadol Devakul. 1989. “Preparation of a Local Strain of Mycoplasma Gallisepticum Antigen for Rapid Serum Plate Agglutionation Test”. Agriculture and Natural Resources 23 (3). Bangkok, Thailand:254-59. https://li01.tci-thaijo.org/index.php/anres/article/view/242302.

Issue

Vol. 23 No. 3 (1989): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.