Purification of Citrus Tristeza Virus

Authors

  • Supat Attathom Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Pattana Srifa Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Ampaiwan Paradornuwat Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Thira Sutabutra Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.

Abstract

Citrus tristeza virus (CTV) was purified from bark tissues of infected lime plants. The purification procedure consisted of extraction of powdered bark tissues, filtration, and virus precipitation by polyethylene glycol. A short Cs2SO4-sucrose cushion step gradient was introduced to further purify the virus. The yield of purified virus was 4 mg/100 g bark tissue. Purified virus suspension composed of abundant threadlike particled and exhibited a UV-absorption spectrum of a nucleoprotein with a A 260/280 of 1.23. The CTV antiserum was effective for virus detection in immune-electron microscopic tests.

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Published

1988-03-31

How to Cite

Supat Attathom, Pattana Srifa, Ampaiwan Paradornuwat, and Thira Sutabutra. 1988. “Purification of Citrus Tristeza Virus”. Agriculture and Natural Resources 22 (1). Bangkok, Thailand:62-64. https://li01.tci-thaijo.org/index.php/anres/article/view/242333.

Issue

Vol. 22 No. 1 (1988): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.