Purification of Citrus Tristeza Virus

ผู้แต่ง

  • Supat Attathom Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Pattana Srifa Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Ampaiwan Paradornuwat Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.
  • Thira Sutabutra Dept. of Plant Patholog, Faculty of Agriculture, Kasetsart Univ.

บทคัดย่อ

Citrus tristeza virus (CTV) was purified from bark tissues of infected lime plants. The purification procedure consisted of extraction of powdered bark tissues, filtration, and virus precipitation by polyethylene glycol. A short Cs2SO4-sucrose cushion step gradient was introduced to further purify the virus. The yield of purified virus was 4 mg/100 g bark tissue. Purified virus suspension composed of abundant threadlike particled and exhibited a UV-absorption spectrum of a nucleoprotein with a A 260/280 of 1.23. The CTV antiserum was effective for virus detection in immune-electron microscopic tests.

ดาวน์โหลด

เผยแพร่แล้ว

1988-03-31

รูปแบบการอ้างอิง

Supat Attathom, Pattana Srifa, Ampaiwan Paradornuwat, และ Thira Sutabutra. 1988. “Purification of Citrus Tristeza Virus”. Agriculture and Natural Resources 22 (1). Bangkok, Thailand:62-64. https://li01.tci-thaijo.org/index.php/anres/article/view/242333.

ฉบับ

ปีที่ 22 ฉบับที่ 1 (1988): January-March

ประเภทบทความ

Research Article

สัญญาอนุญาต

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