Production of Vieus-Free Sugarcane by Tissue Culture

Authors

  • Rongrong Visessuwan The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Wichai Korpraditskul The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Supat Attathom The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Supaporn Klinkong The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.

Abstract

Sugarcane plants of the three commercially varieties namely F140, Q83 and Supan I were produced free from sugarcane mosaic virus by the apical meristem culture. Shoot tips, 0.2-0.5 mm. in size, were cultured on MS medium supplemented with 3 mg/L 2,4-D and 15% (v/v) coconut water. Yellowish-white callus developed within 2 months. Plantlets were regenerated from calli cutured on MS medium with 1 mg/L 2,4-D and 15% (v/v) coconut water. The suitable medium for root induction in all cultivars was the MS or MS with 1 mg/L IBA. Detection of sugarcane mosaic virus (SCMV) in plantlets or individual plants by ELISA showed negative result suggesting that they were virus free.

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Published

1988-12-31

How to Cite

Rongrong Visessuwan, Wichai Korpraditskul, Supat Attathom, and Supaporn Klinkong. 1988. “Production of Vieus-Free Sugarcane by Tissue Culture”. Agriculture and Natural Resources 22 (5). Bangkok, Thailand:30-36. https://li01.tci-thaijo.org/index.php/anres/article/view/242563.

Issue

Vol. 22 No. 5 (1988): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.