Production of Vieus-Free Sugarcane by Tissue Culture

ผู้แต่ง

  • Rongrong Visessuwan The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Wichai Korpraditskul The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Supat Attathom The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.
  • Supaporn Klinkong The Central Laboratory and Greenhouse Complex, KURDI, Kastsart University, Kamphaengsaen Campus, Nakorn Pathom, Thailand.

บทคัดย่อ

Sugarcane plants of the three commercially varieties namely F140, Q83 and Supan I were produced free from sugarcane mosaic virus by the apical meristem culture. Shoot tips, 0.2-0.5 mm. in size, were cultured on MS medium supplemented with 3 mg/L 2,4-D and 15% (v/v) coconut water. Yellowish-white callus developed within 2 months. Plantlets were regenerated from calli cutured on MS medium with 1 mg/L 2,4-D and 15% (v/v) coconut water. The suitable medium for root induction in all cultivars was the MS or MS with 1 mg/L IBA. Detection of sugarcane mosaic virus (SCMV) in plantlets or individual plants by ELISA showed negative result suggesting that they were virus free.

ดาวน์โหลด

เผยแพร่แล้ว

1988-12-31

รูปแบบการอ้างอิง

Rongrong Visessuwan, Wichai Korpraditskul, Supat Attathom, และ Supaporn Klinkong. 1988. “Production of Vieus-Free Sugarcane by Tissue Culture”. Agriculture and Natural Resources 22 (5). Bangkok, Thailand:30-36. https://li01.tci-thaijo.org/index.php/anres/article/view/242563.

ฉบับ

ปีที่ 22 ฉบับที่ 5 (1988): January-December

ประเภทบทความ

Research Article

สัญญาอนุญาต

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