cDNA Probe for Grapevine Yellow Speckle Viroid Detection

Authors

  • Pattama Hannok Center for Agricultural Biotechnology, Kasetsart University.
  • Kanuangnit Reanwarakorn Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

grapevine, RT-PCR, GYSVd-1, viroids, cDNA probe

Abstract

Isolates of grapevine yellow speckle viroid (GYSVd-1) were found in Saraburi province, having 366 and 367 bp in size, and showed 96% sequence homology with Australian GYSVd-1. GYSVd cDNA probe was proven to be sensitive and highly specific to GYSVd-1. This cDNA probe could detect very
low amount of the viroid even in the symptomless samples. Thus, GYSVd-1 cDNA probe would be very useful for plant quarantine or viroid free certification schemes, since the large number of samples could be tested for viroid infection with high accuracy.

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Published

2005-03-31

How to Cite

Pattama Hannok, and Kanuangnit Reanwarakorn. 2005. “CDNA Probe for Grapevine Yellow Speckle Viroid Detection”. Agriculture and Natural Resources 39 (1). Bangkok, Thailand:46-52. https://li01.tci-thaijo.org/index.php/anres/article/view/243284.

Issue

Vol. 39 No. 1 (2005): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.