Sonication-Assisted Agrobacterium-mediated Gene Transformation of Oil Palm Secondary Somatic Embryo

Authors

  • Sureerat Yenchon Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112, Thailand.
  • Sompong Te-chato Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112, Thailand.

Keywords:

sonication, Agrobacterium, oil palm, secondary somatic embryo, hygromycin

Abstract

The infl uence was investigated of the hygromycin concentration on the survival rate of oil palm secondary somatic embryos (SSEs) and sonication-assisted Agrobacterium-mediated transformation (SAAT). The results showed that 20 mg.L-1 hygromycin could completely inhibit growth of SSEs, suitable for selection of transformed SSEs. The transient ß-glucuronidase (gus) expression was affected by the different period of SAAT which was signifi cantly enhanced by increasing the period of SAAT. However, the longer exposure to SAAT caused a decrement in the frequency of plantlet regeneration. The highest transformation effi ciency was obtained when the SSEs were sonicated for 5 min. Polymerase chain reaction and Southern blot hybridization analysis confi rmed the presence of the reporter genes in the transformed plantlet genome.

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Published

2015-06-30

How to Cite

Yenchon , Sureerat, and Sompong Te-chato. 2015. “Sonication-Assisted Agrobacterium-Mediated Gene Transformation of Oil Palm Secondary Somatic Embryo”. Agriculture and Natural Resources 49 (3). Bangkok, Thailand:319-26. https://li01.tci-thaijo.org/index.php/anres/article/view/243575.

Issue

Vol. 49 No. 3 (2015): May-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.